目标：研究伏隔核（Nucleus accumbens , NAc）在焦虑情绪中的神经回路调控机制，理解DISC1转基因精神疾病动物模型焦虑样行为变异的机制。本项目将以Cre重组酶转基因小鼠及DISC1突变的转基因小鼠为研究对象, 综合运用光遗传、化学遗传学等方法，结合自由活动的动物光刺激-电生理记录与行为检测等手段: 1）解析NAc脑区不同类型神经元和编码焦虑行为的关系；2）研究NAc脑区局部场电位中的theta节律与焦虑行为之间的相关性；3）分析DISC1转基因动物NAc脑区不同类型神经元的放电特征改变与焦虑行为变异的关系；4）探索胶质细胞和神经元之间的相互作用对NAc脑区局部和输出神经网络的影响，并进而参与病理性焦虑行为调控的可能机制。通过本项目的研究，我们将能够进一步理解大脑对负性刺激处理的神经回路机制，加深对精神疾病中焦虑等情绪失调的精细环路的理解。

Objectives: to study the role of NAc in physiological anxiety-related behaviour and to understand the neuronal circuitry mechanism for the pathological anxiety in DISC1 transgenic mice. .Methods and results: in the present study, the Cre-transgenic mice and DISC1 transgenic mice would be employed, and a combination of optogenetics, chemogenetics and in vivo multi-channel recordings in freely moving animals would also be performed. We would 1) to dissect the neuronal types for coding the safe and threaten information; 2) to study the correlation between theta power in the NAc and the firing patterns of anxiety-related neurons; 3) to demonstrate the different firing patterns of the NAc in DISC1 mice for coding anxiety-related behaviour; 4) to explore the possible role of the glia cells in modulation the abnormal anxiety-related behaviour in DISC1 mice. .Conclusions: through this project, we would like to understand the mechanism for the brain in processing the threaten information and to further interpret the pathophysiological mechanism for the negative emotion in mental disorders.