设施栽培下CO2加富能提高蔬菜的产量及品质，然而，CO2环境-设施作物-病原生物的互作关系及机制还远不清楚。本项目拟以番茄为材料，利用人工控制环境（CE）及开放式空气CO2加富（FACE）的方法进行CO2控制，采用叶绿素荧光成像、台盼蓝染色等方法，检测典型病毒、细菌、真菌不同类型病原胁迫对番茄作物的影响，明确CO2环境-作物-病原生物间互作关系；利用突变体、基因沉默及过量表达植株，研究CO2加富环境下SA、JA/ET不同抗性路径间的交叉对话，解析CO2升高环境下作物-病原生物的互作机制；在此基础上，探讨具SA、JA/ET抗性路径间cross-talk调控活性的烷基酰胺脂类物质对CO2加富下病原胁迫抗性的影响及机制。研究可望对CO2环境-作物-病原互作机制与调控有一个较为全面清晰的认识，对于设施农业生产实践中利用CO2加富的优势而规避其劣势，具有十分重要的理论和实践意义。

CO2 enrichment has become one of the main effective ways for enhancing yield and quality for greenhouse vegetables. However, the effects of elevated CO2 on plant-pathogen interaction and the mechanism involved remain largely unclear. In the present project, tomato plants will be CO2 enriched using controlled environment (CE) and free-air CO2 enrichment (FACE) facilities, the effects of elevated CO2 on interactions between tomato and typical virus, bacteria and fungi pathogens which cause serious disease on tomatoes will be evaluated using chlorophyll fluorescence imaging method and trypan bule staining. Mutants and gene silencing tomato plants will be used to investigate the crosstalk between major SA, JA/ET related defense pathways during the interaction. Meanwhile, the alleviatory effects of natural active alkamides which can regulate SA-JA/ET crosstalk will also be examined. These results will not only give insight into the effects of elevated CO2 on plant-pathogen interactions in tomato crops, but also be necessary to supply theoretical basis for enhancing the vegetables productions during CO2 enrichment.