Bt Cry毒素对蜜蜂中肠上皮细胞电生理研究

Bt cry1Ab和cry1Ac基因在转基因作物中应用最广。新型杀虫蛋白基因cry1Ah基因是中国农业科学院从Bt菌株BT8中鉴定克隆的，其编码蛋白对鳞翅目害虫具有强毒力，尤其对亚洲玉米螟（Ostrinia furnacalis）的毒力强于目前使用的cry1A类基因。转cry1Ah基因抗虫玉米具有很好的应用前景。本项目拟通过膜片钳技术研究Cry毒素对西方蜜蜂Apis mellifera上皮细胞离子通道的影响，探讨Bt毒素对蜜蜂影响的作用机理，为Bt毒素对靶标或非靶标生物的毒性研究提供依据。

Bt cry1Ab and cry1Ac genes were used widely in transgenic crops. The cry1Ah gene was one of the novel insecticidal genes cloned from Bacillus thuringiensis isolate BT8 which exhibited high toxicity against lepidopteran larvae. Cry1Ah protein showed higher toxicity to Asian corn borer (Ostrinia furnacalis) than that of any other cry1A genes. The cry1Ah gene was a candidate gene for insect resistant transgenic corn research. In this study, a whole-cell patch-clamp technique was used to study effects of Bt Cry toxin on honey bee Apis mellifera midgut epithelium ion channels and explore the mechanisms of the effect of Bt toxin on honey bee in order to provide a basis of Bt toxin to target or non-target organisms risk assessment.