人体是一个非平衡的开放系统，又是一个高级的耗散结构，在不断与内外环境进行物质、能量等各种信息交换时，用负熵克服体内的正熵，维持机体的有序态。绝经后骨质疏松症是因雌激素水平下降引起Treg/Th17介导的骨免疫功能增强，导致骨偶联失衡，破骨细胞的骨吸收超过成骨细胞的骨形成，引发体内的正熵增加，从而影响机体的有序态。课题组前期研究发现绝经后骨质疏松症肾阳虚证素积分明显升高，提示肾阳虚可能是影响体内正熵变的重要因素之一，故本研究基于肾虚骨痿理论，以熵变理论为切入点，以绝经后骨质疏松症患者为研究对象，采用流式细胞术、悬液芯片、Real time PCR、Western blot等技术，观察绝经后骨质疏松症肾阳虚证熵变与Treg/Th17细胞数量及骨免疫相关因子、Foxp3、RORγt mRNA与蛋白表达的相关性，探讨肾阳虚证熵变与骨免疫失衡的内在关系，为阐明肾阳虚证的形成机制提供研究基础。

Human body, a non-equilibrium open system and an advanced dissipation structure, is maintained the orderly state and continuous exchange of material, energy and all kinds of information between internal and external environment by negative entropy, which would overcomes the internal positive entropy. Postmenopausal osteoporosis leads to increase the positive entropy of body and then affects the body's orderly state, because estrogen withdrawal enhances the function of Treg/Th17 mediated bone immune. It also would cause bone coupling imbalances and bone resorption of osteoclast surmounting bone formation of osteoblast. Our previous studies suggest that the syndrome element integral of postmenopausal osteoporosis kidney-yang deficiency increased significantly, which indicates that kidney-yang deficiency may be an important factor in the formation of the body's positive entropy. Therefore, the Guwei due to kidney deficiency and entropy theory will be very useful for the further research of the formation of kidney-yang deficiency. With postmenopausal osteoporosis patients as objects, we will detect Treg/Th17 cell number, mRNA, protein expression of bone immune related factors, Foxp3 and RORγt using flow cytometry, suspension array, Real time PCR and Western blot, and thus explore the relation of the entropy change in kidney deficiency and bone immune imbalance. The study will provide fundamental knowledge for the mechanism of the kidney-yang deficiency formation.