蓝色素作为三大基本色素之一，因制备资源稀少而十分稀缺，寻求微生物源食用蓝色素有望突破其来源瓶颈。课题组前期发现1株胞外高产脂溶性和水溶性两类蓝色素的脱叶链霉菌A1013Y，已从脂溶性组分发现一个安全、新型吡喃并萘醌类蓝色化合物。本项目拟在此基础上利用各种色谱、波谱手段对剩余组分和未研究的水溶性蓝色组分的化学成分（包括微量成分）进行系统分离纯化和结构鉴定，全面解析蓝色素化学组成；在确证蓝色单体安全性基础上，利用基因组测序等现代分子生物学技术挖掘蓝色素生物合成关键酶基因并预测代谢途径；通过异源共表达代谢途径关键酶基因及对关键化合物结构信息解析验证关键酶基因功能，最终明确蓝色素的生物合成途径。本项目的成功实施有望实现脱叶链霉菌A1013Y代谢产物中蓝色素产量提高和组分优化，为在基因水平通过组合生物学获得更多安全新型食用蓝色素奠定理论基础，为微生物发酵制备食用蓝色素的产业化提供技术支撑和理论依据。

As one of the three basic pigments, natural edible blue pigment is very scarce because of the scarcity of resources. Our research group found that Streptomyces exfoliatus A1013Y can produce two kinds of blue pigment including extracellular fat-soluble and water-soluble. From the fat-soluble components, a safe and novel pyranonaphthoquinone compounds has been found. Based on these research results, the current project will continue perform purification and structure identification of chemical compositions (including trace elements) in the fat-soluble and water-soluble section by using various means of chromatogram and spectrum; On the basis of confirmation of monomer safety, exploring key enzyme biosynthesis gene of the blue pigment and prediction of metabolic pathways using genomic sequencing modern molecular biology technology; By heterologous expression function of key enzyme genes and analysis of key compounds structure information in metabolic pathways, a clear blue pigment biosynthesis will be clarified. The successful implementation of this project is expected to achieve improved and group S. exfoliatesA1013Y metabolites in blue pigment production optimization, for the follow-up at the gene level through a combination of biological, more safety of edible blue pigment and lay the theoretical foundation, to provide technical support and theoretical basis for the preparation of microbial fermentation of edible blue pigment industry.