猪链球菌是能够引起如心内膜炎、关节炎、脑膜炎和急性出血性败血症等多种症状的一类重要的人猪共患病病原菌，它对养猪业带来严重的损失，同时给人带来威胁。自2005年我国四川发生人猪共患链球菌病以来，链球菌致病机制及防控的研究已经成为研究热点，特别是其生长、繁殖及毒力调控等起着重要作用的信号转导系统备受关注。本实验室前期研究发现具有信号转导作用的猪链球菌2型分子伴侣trigger factor基因缺失后能够引起细菌的毒力和耐受性均显著降低，但是其对细菌毒力及耐受性调控的分子机制仍不清楚。本研究拟通过染色质免疫共沉淀测序、基因芯片以及蛋白质组学等体内、体外多种技术和手段，从分子水平上系统的研究阐述trigger factor 调控猪链球菌2型基因转录与表达，影响致病性和耐受性的分子机制，为猪链球菌2型致病机理的研究和疾病的预防奠定基础。

Streptococcus suis serotype 2 is an important zoonotic pathogen that causes serious diseases such as meningitis, septicemia, endocarditis, arthritis and septic shock in pigs and humans.It brought serious losses to pig breeding industry and posed a serious threat to people at same time. Reasearch about the mechenism and prevention of streptococcus suis 2 has became a research focus in the fields from 2005 an unprecedented epidemic in Sichuan province of China. Signal transduction system which can regulat its growth、reproduction and virulence has been payed close attention. Trigger factor is an important molecular chaperone which involved in protein export and signal transduction. Deletion of the trigger factor gene can cause lower virulence and tolerance of streptococcus suis 2 in our preliminary study, but the regulative mechenism is still unclear.　In the research many kinds of technologies and methods such as chromatin immunoprecipitation sequencing、gene chips and protemics were used.System studied and expounded the molecular mechanism of trigger factor , which control the gene transcription and expression lead to the depression of virulence and tolerance of streptococcus suis 2. The research will lay the foundation for the pathogenesis research and disease prevention of streptococcus suis 2.