睡眠觉醒紊乱严重影响生活质量，已成为社会和医学界共同关注的难题之一，目前仍缺乏理想应对策略。中医学认为昼夜节律的运行机制在于营卫之气的循行，阴阳跷脉受营卫之气影响而调节睡眠。针刺调节睡眠有着悠久的历史和临床积累，本课题组前期运用电针阴阳跷脉“申脉、照海穴”获得了良好临床疗效，研究同时显示其作用可能与调节“褪黑素(MEL)及其受体”的表达相关。下丘脑视交叉上核(SCN)生物节律基因Clock/Bmal1-Pers/Crys通路一直是睡眠节律调控关注的热点，为进一步深入研究，我们将以模式动物为研究对象进行电针干预，并利用高自动化的睡眠生物解析系统与时间生物学的分析方法，从MEL及其受体、生物节律基因及其通路Clock/Bmal1-Pers/Crys在SCN中的昼夜节律变化规律进行研究，以期系统阐释电针调节睡眠觉醒紊乱的分子作用机制，为其在临床推广与应用提供科学的理论和实验依据。

Sleep-wake disorders seriously affect quality of life, has become one of the social problems of common concern and the medical profession, There are still no ideal coping strategies. Chinese medicine believes that the circadian rhythm of the operating mechanism of the ying and wei Circulation, yin and yang qiao mai affected by ying and wei Circulation to regulating sleep. Acupuncture has a long history and clinical accumulated sleep, Our previous use of acupuncture yin and yang qiao mai "Shen ma、zhao hai” was a good clinical efficacy, The study also showed its effect may be "melatonin (MEL) and its receptor," the expression and regulation of the relevant. Hypothalamic suprachiasmatic nucleus (SCN) of biological circadian gene Clock / Bmal1-Pers / Crys pathways regulating sleep rhythm has been the focus of attention, For further study, we will conduct a model animal for the study acupuncture intervention, And the use of highly automated analytical system sleep biological analytical methods and Chronobiology, Clock genes and pathways regulating from its receptor MEL, Clock / Bmal1-Pers / Crys pathways in the circadian rhythm of SCN research, Systematic Interpretation of electroacupuncture regulating the sleep-wake disorders molecular mechanism of action, Provide scientific theory and experimental evidence and clinical application.