以神经血管单元（NVU）为基础的大脑微环境的修复，对脑缺血后的神经恢复起着重要作用。脑缺血伴随血脑屏障（BBB）等微环境结构与功能破坏，其中血管周细胞是微环境中的重要成分。脑缺血后，周细胞可在微环境修复中发挥多种功能作用，包括促进血管生成和BBB的稳定性、以及多分化潜能。我们前期的研究表明，白藜芦醇（Resvertrol，Res）预处理可促进周细胞的存活，使得PDGFRβ表达上调。本课题拟从脑缺血后Res预处理为前提，以NVU微环境中的周细胞之结构与功能为重点，采用动物模型、从体内、外两个层面、以及细胞培养及形态学、分子生物学及神经功能学等多种技术手段，探讨Res在脑缺血后通过调节周细胞的存活，并促使血管生成、BBB重建及多潜能分化之机制。旨在从分子生物学层面阐明脑缺血后Res的脑保护作用，并为脑缺血后促进脑微环境的重建、为加快神经修复提供新的治疗策略。

The structure and function of cerebral microenvironment based on the neurovascular unit (NVU) is important for the neuronal recovery after cerebral ischemia. The structure and function of blood brain barrier (BBB), which is critical components of the NVU, are impaired during cerebral ischemia. It has been recognized that pericyte plays important roles for the recovery of cerebral microenvironment, such as promoting angiogenesis, stability of BBB, and multi-differentiation potentials. Our previous study demonstrated that resveratrol pre-treatment in cerebral ischemia mice model promoted survival of pericyte, up-regulation of PDGFRβ. In this project, in vivo and in vitro studies will be performed to focus on the effect of Resveratrol pre-treatment on the structure and function of pericytes after cerebral ischemia. The mechanisms of Resveratrol promoted pericyte survival, angiogenesis, re-construction of BBB, and multi-differentiation potential of pericyte will be investigated by using animal model, cell culture, brain slice culture, and morphology, molecular biology, functional neurology techniques. The results will reveal more neuroprotective mechanisms of resveratrol for cerebral ischemia from novel perspectives, and provide promising novel strategies to improve the efficacy of stem cell therapy for cerebral ischemia through promoting the re-construction of cerebral microenvironment.