烧伤是物理、化学、放射等诸多因素作用于机体所造成的一种极其复杂的外伤性疾病，创面愈合仍是烧伤治疗中的一大难题，寻找、发现和鉴定参与烧伤创面愈合的相关分子，并阐明其作用机制意义重大。BPC-157是一个由15个氨基酸组成的多肽，本课题组前期研究发现BPC-157具有良好的促进烧伤创面愈合的作用，可显著增强内皮细胞的增殖、迁移及小管形成等生物学功能。上述结果提示，BPC-157可考虑作为治疗烧伤创面愈合的候选药物，但其与靶细胞作用的首触分子尚不清楚。本课题拟在前期工作基础上，以探明BPC-157的相互作用分子为突破口，综合利用亲和纯化、串联质谱、RNAi、基因转染和免疫荧光等实验技术，系统研究能够与BPC-157相互作用的分子及其对内皮细胞的影响，初步阐明这种相互作用在烧伤创面愈合过程中的作用，该研究既为烧伤皮肤修复机理提出新认识，又为阐明BPC-157促进创面愈合的药物作用靶点提供依据。

Burns is a very complicated traumatic disease which is caused by physical, chemical, radiation and many other factors affecting on the body. The treatment of burn wound healing is still a big problem, so searching, detecting and identifying related molecules involving in burn wound healing, and clarifying mechanism underlying is of great significance. The body protection compound 157 (BPC-157) is a composed of 15 amino acid polypeptide. Our previous studies have found that the BPC-157 has good promoting burn wound healing effect and can significantly enhance proliferation, migration and tube formation of endothelial cell. These results suggest that BPC-157 can be a candidate for therapeutic agents in the treatment of burn wound healing. However, the specific molecular mechanism is unclear. Based on previous data, we plan to take the studies of BPC-157 interaction partners as a breakthrough, utilize affinity purification, tandem mass spectrometry, RNAi, gene transfection, and immunofluorescence to screen the BPC-157 binding-partners, study its influence on endothelial cells and preliminary to elucidate the role of the interaction in burn wound healing process. This study is to put forward a new understanding for repair mechanism of burned skin, and to provide the basis for elucidating the BPC-157 drug targets of promoting wound healing.