从lncRNA MGC-Mirg与miRNA-377-3p构成的ceRNA网络探讨荣筋拈痛方调控内质网应激延缓骨关节炎软骨退变的机制

骨关节炎严重影响中老年人的生命健康。而软骨细胞内质网应激是加剧骨关节炎病理退变的重要事件。源于清代宫廷秘方之荣筋拈痛方具有补肝肾、祛风湿之效，临床治疗骨关节炎具有良效。课题组前期研究证实，荣筋拈痛方可调控内质网应激反应，改善骨关节炎软骨组织结构，但其是否从内质网应激关联的ceRNA调控网络发挥作用的机制尚需进一步阐明。由此提出假说：荣筋拈痛方通过调控LncRNA MGC-Mirg与miRNA-377-3p构成的与内质网应激关联的ceRNA网络，进而延缓骨关节炎的病理进程。围绕该假说，本课题以骨关节炎小鼠模型和shRNA Mirg转染的软骨细胞为研究对象，运用基因编辑、悬液芯片、双荧光素酶法、荧光双标染色等技术进行验证，以期为该方临床应用提供实验依据。

Osteoarthritis seriously harms the life and health of middle and old age residents. Endoplasmic reticulum stress (ERS) has been involved in the pathological process of osteoarthritic, endangers homeostasis in chondrocytes. The Rongjin Niantong decoction derived from the Qing dynasty palace secret recipe has the effect of tonifying the liver and kidney, expelling wind and damp, which has good effect on the clinical treatment of osteoarthritis. Previous studies from our team have confirmed that Rongjin Niantong decoction can improve the cartilage structure of osteoarthritis by regulating ERS. However, the mechanism of whether it acts from the ceRNA regulatory network related to the ERS still needs to be further clarified. So we put forward a hypothesis that Rongjin Niantong decoction delays the pathological process of osteoarthritis by affecting ceRNA regulatory network formed by LncRNA MGC-Mirg and miRNA-377-3p related to ERS. Around this hypothesis, in order to further study its mechanism which of Rongjin Niantong decoction delays the pathological process of osteoarthritis by affecting ceRNA regulatory network, gene editing, suspension chip, double luciferase method, fluorescent double standard staining and other technologies will be applied in the mouse model of osteoarthritis and LncRNA MGC-Mirg shRNA chondrocyte, so as to provide experimental basis for the clinical application of this prescription.