乳腺癌是女性恶性肿瘤死亡的主要原因，三阴乳腺癌具有高侵袭性，极易复发转移。上皮间质转化(EMT)是乳腺癌细胞转移的重要途径，PI3K/AKT/ mTOR信号通路在肿瘤细胞增殖、血管生成、转移以及放化疗抵抗中起重要作用，通路过度激活后可发生EMT，导致肿瘤的形成和转移。运用中医“护场”理论，课题组前期发现薯蓣丸在调节肿瘤免疫、降低ki-67等方面有较好作用。本课题基于PI3K/AKT/mTOR信号转导通路，运用RT q-PCR、WB等技术检测通路及EMT相关的PI3K、AKT、mTOR、E-cadherin 、N-cadherin 、Vimentin、MMP-2等基因与蛋白的表达，观察薯蓣丸对荷瘤裸鼠肿瘤大小、肿瘤组织的结构形态以及全身转移情况的影响。探究薯蓣丸与P13K/Akt/mToR信号通路、EMT、肿瘤转移之间的关系，寻找有效防止肿瘤转移的药物，进一步验证“护场”理论。

Breast cancer is the main cause of death in female malignant tumors，of which triple-negative breast cancer is highly invasive and highly susceptible to recurrence and metastasis. Epithelial-mesenchymal transition (EMT) is an important pathway of breast cancer cell metastasis. PI3K/AKT/ mTOR signaling pathway plays an important role in tumor cell proliferation, angiogenesis, metastasis, and chemoradiation resistance. EMT can occur after excessive activation of the pathway, leading to tumor formation and metastasis. Based on the "Hu-Chang" theory, our research group has found that Shuyu Pill has a good effect on tumor immunity and reduction of ki-67 in the early stage. Based on the PI3K/AKT/mTOR signaling pathway, this study will use RT q-PCR, WB and other techniques to detect the pathway and EMT-related genes and proteins,such as PI3K, AKT, mTOR, E-cadherin, N-cadherin, Vimentin and MMP-2 etc. to observe the effect of Shuyu Pill on tumor size, tumor tissue structure and systemic metastasis in in breast cancer mice. To explore the relationship between Shuyu Pill and P13K/Akt/mToR signaling pathway, EMT and tumor metastasis in order to find drugs that can effectively prevent tumor metastasis, and further verify the theory of “Hu-Chang”.