基于毒瘀理论的解毒活血汤调控TGF-β/SMAD信号通路治疗III型前列腺炎的作用机制研究

III型前列腺炎是由多种炎性基因共同作用的自身免疫性炎性疾病，TGF-β/SMAD信号通路的异常活化与免疫炎症反应密切相关。基于“毒瘀”理论的解毒活血汤治疗III型前列腺炎临床疗效显著，然而其作用机制尚未完全阐明。我们前期研究发现TGF-β/SMAD信号通路对前列腺炎免疫应答和炎症反应过程具有重要的调节作用，提示TGF-β/SMAD介导免疫炎症反应，可能是其治疗前列腺炎的重要机制。本课题拟运用RT-PCR、Western blot、免疫组化、IHC、TUNEL染色法、流式细胞Annexin-V-FITC/PI双染法、透射电镜等技术，研究解毒活血汤对III型前列腺炎免疫炎症反应的抑制作用和对TGF-β/SMAD信号通路的影响。研究结果将有助于从分子、细胞、组织形态和整体水平阐明解毒活血汤治疗III型前列腺炎的分子机制和作用靶点，为临床科学用药提供实验依据。

Type III prostatitis is one kind of autoimmune inflammatory disease which is caused by the interaction of multiple inflammatory genes. Aberrant activation of TGF-β/SMAD is strongly associated with immuno-inflammatory responses. Jieduhuoxue decoction, coming from toxic stasis theory, has been demonstrated to be clinically effective in the treatment of type III prostatitis. However, the underlying mechanism of its anti-inflammatory activity remains to be further elucidated. Our previous studies found that TGF-β/SMAD signaling pathways play key role in regulating the immune response and inflammatory response, suggesting that Jieduhuoxue decoction may exert its anti-inflammatory function via regulating TGF-β/SMAD signaling pathway. To further investigate the precise mechanism of its anti-inflammatory activity,in present project we will evaluate the inhibitory effect of Jieduhuoxue decoction on the process of immuno-inflammatory responses by flow cytometry, transmission electron microscopy Western blot, Immunohistochemistry assay and so on. In addition, we will determine Jieduhuoxue decoction’s effects on the activation of TGF-β/SMAD/ signaling pathway. Our findings in this project will further elucidate the mechanism whereby Jieduhuoxue decoction treats Type III prostatitis.