酶脱氧SI-ATRP可控构筑Geminized两亲聚合物刷抗菌功能表面研究

随着耐药性超级细菌在全球范围内的快速传播，迫切需求具有更新换代意义的“超级”抗菌功能表面。由于双功能聚合物刷表面抗菌机理认识的不足及制备方法的限制，新型高效抗菌表面材料的设计开发及相关基础研究至今仍徘徊不前。本项目拟采用酶脱氧SI-ATRP法可控构筑具有超高电荷密度的双功能Geminized两亲聚合物刷抗菌表面，借助QCM-D/LSPR联用技术，精确分析聚合物刷原位生长曲线，获得聚合物刷表面接枝过程的实际质量变化，实现对聚合物刷生长过程的实时监测与精准调控。揭示聚合物刷接枝密度、表面电性及亲/疏水性与杀菌、释菌性能的构效关系。阐明细菌外膜与功能表面之间的相互作用方式和本质，获得功能表面高效抗菌性能的关键影响因素，为新型高效医用抗菌材料的设计与制备提供新的思路。

With the rapid spread of drug-resistant superbugs around the world, there is an urgent need for a new generation of "super" antibacterial functional surfaces. The antibacterial mechanism and preparation methods of dual-functional polymer brush surfaces are lacking, leading to the stagnant of the design and development as well as the relative fundamental researches of novel high-efficiency antibacterial surface materials. Herein, a dual-functional geminized amphiphilic polymer brush antibacterial surface with ultrahigh charge density will be constructed controllably by enzymatically deoxygenated SI-ATRP, and the in situ growth curves will be detected and analyzed accurately by QCM-D/LSPR technology. The actual adsorption mass of polymer brushes will be obtained, to realize the real-time monitoring and precise regulation of growth process. The relationship between the graftting density, surface charge density, hydrophilic/hydrophobic properties of polymer brush and its bactericidal and bactericidal properties will be revealed. Moreover, the interaction mechanism between bacterial outer membrane and functional surfaces will be explored, and the critical factors influencing high-efficiency antibacterial performance of functional surfaces will be finally obtained. This study will provide an innovative method for designing and preparing novel high-efficiency medical antibacterial materials.