石墨烯是现今科技与工业界最受瞩目的纳米材料之一。为从根本上控制其进入实际环境后可能发生的各种危害，本项目选择斑马鱼、鲫鱼等鱼类以及沙蚕为水生模式生物，探索石墨烯纳米颗粒诱发水生模式生物的代谢毒性规律，即在石墨烯纳米颗粒暴露和诱导下这些典型水生模式生物体重增加的相关毒性、心血管损伤、肠道微生物群落变化与炎症反应以及神经毒性方面的变化，同时通过气相色谱质谱联用技术测定、分析石墨烯纳米材料诱导水生模式生物体内代谢组学的变化，并建立其相关关系；使用基因组学、蛋白质组学和代谢组学相关技术对水生模式生物的基因、蛋白和代谢物及其同路进行研究、筛选，探究基因表达变化对蛋白的影响和蛋白的变化对代谢通路的作用，结合各项毒性指标，通过查阅相关文献和验证实验，详细阐释石墨烯纳米材料对水生模式生物相关毒性效应的产生机理，为探寻以石墨烯为基础的新兴产业的发展以及生态安全性评价方法提供基础数据和相关科技支撑。

Graphene is one of the most high-profile nanomaterials in the modern science and technology and the industrial community. In order to radically control various hazards from graphene nanomaterials into the actual environment, metabolic toxicology induced by graphene nanoparticles acting on zebrafish, crucian carp and clamworms selected as typical aquatic model organisms, namely, weight-increasing relevant toxicity, cardiovascular damage, intestinal microbial community changes, inflammatory responses and neurotoxicity of these typical aquatic model organisms under the condition of exposing to graphene nanoparticles, will be tentatively explored. In the meantime, by using the gas chromatography / mass spectrometry (GC/MS) technique, changes in metabolomics of aquatic model organisms induced by graphene nanomaterials will be analyzed and determined, and the correlation relationships between metabolomics changes and graphene nanoparticles will be established. Genes, proteins, and metabolites, and the same way of the aquatic model organisms will be studied and screened out, in particular, acting mechanisms of relevant toxic effects of the aquatic model organisms by graphene nanomaterials will be revealed using genomics, proteomics and metabonomics technology, and influences of gene expression changes on proteins and the action of protein changes on the metabolic pathway will be explored while combined with various toxic indexes, relevant references and confirmatory experiments, thereby providing with basic data and relevant scientific and technological support for the development of emerging industries based on graphene nanomaterials and setup of ecological safety and risk evaluation methods.