真核细胞在遇到逆境时会形成一种所谓应激颗粒（SGs）的细胞质信使RNA蛋白质复合体。在动物中大量研究表明SGs是逆境下RNA的分选位点及信号传递中心，对细胞的逆境适应和保护起着极为重要的作用。在植物中已报道在热激、氧化胁迫下形成与哺乳动物中类似的SGs，但对SGs的生理功能及形成机制还缺乏了解。我们前期工作表明盐胁迫下植物细胞中也形成SGs,而且RNA结合蛋白Tudor-SN是SGs的一个组分，在盐胁迫下Tudor-SN结合GA20ox3 mRNA，积聚到SGs中提高了mRNA的稳定性，为SGs可能参与植物逆境适应提供了实验证据。在此基础上，本项目将通过细胞生物学及分子生物学等手段进一步研究可能的SGs成核关键组分（RBP47、UBP1）以及Tudor-SN对SGs形成的影响，进而阐明SGs形成在植物盐逆境适应中的生理意义，为阐明植物适应盐逆境的机制提供新内容。

Stress granules(SGs) are cytoplasmic mRNA–protein aggregates formed upon stress in eukaryote. In mammals, SGs have been shown to function as mRNA triage centers and signaling centers, therefore play important roles in enhancing resistance to stress and protecting cells. It has been reported that plant cells contain SGs that are conserved from mammals under heat shock and oxidative stress, but the function of SGs formation and the mechanism of SGs assembly remain poorly understood. Our previously works showed that salt stress induced SGs formation, and a RNA binding protein Tudor-SN was a novel component of SGs. Tudor-SN bound GA20ox3 mRNA and accumulated to SGs to enhance the stability of GA20ox3 mRNA. Our work provided the first evidence that SGs may play important roles in plant stress adaption. On the basis of this study, we will investigate the roles of putative SGs-nucleated key proteins (RBP47 and UBP1) and Tudor-SN on SGs assembly, and furthermore the physiological functions of SGs formation under salt stress. This study will provide new insight for adaption mechanism of plant response to salt stress.