基于“肺与大肠相表里”从SP-D表达及相关信号通路功能状态探索燥邪致病的分子机制

西北燥证是我国干旱半干旱区燥邪所致之复合型中医证候，罹患该证是西北地区人群亚健康及常见病的诱因和加重因素，探索燥邪致病机制有助于科学防治燥证。项目前期已建立并验证燥邪实验室模拟方案，使秋燥与西北燥邪微观致病机制的比较研究成为可能；证实了燥邪引发肺、气道SP-D表达增强、机体细胞免疫功能亢进，但AQP-5表达并未发生改变；且虽秋燥和西北燥邪微观致病机制不尽相同，但健康机体对此具有习服能力。正虚是燥邪致病的内因，本研究以维持肺泡表面气液平衡且具固有免疫功能的SP-D为正气卫外的标志物，拟运用iTRAQ和生物信息学技术分析正常组、秋燥组、西北燥邪组、SP-D KO秋燥组、SP-D KO西北燥邪组大鼠肺、降结肠的差异表达蛋白，在比较秋燥与西北燥微观致病机制中寻找更有价值的线索，为阐发基于燥邪致病的肺与大肠相表里及外燥证分子机制提供依据，也为基于证实质探索干旱半干旱区中医药防治外燥证做准备。

Northwest Dryness Syndrome(NDS) is the combine TCM syndrome causing by northwest dryness evil(climatic factors of arid and semi-arid area),NDS suffering is the risk and aggravating factor of the pathogenesis of many kinds of special high incidence diseases or sub-health in northwest China,study of dryness evil pathogenicity essence will help scientific prevention and treatment of dryness syndrome.. Our former research have establish and verify dryness laboratory simulation program that made it possible to compare the microcosmic pathogenesis of autumn dryness evil(ADE) and northwest dryness evil(NDE),we have verified that dryness evil induced SP-D enhanced expression in lungs and airways,and hyperactive immune function of cells in the body, but the expression of AQP-5 was not changed.And although the microcosmic pathogenesis of ADE and NDE is not the same, the healthy organism has the ability to adapt to it.. Zhengqi deficiency is the internal cause of the disease caused by external pathogenic factors.In this study, SP-D, which maintains the gas-liquid balance on the surface of alveoli and has inherent immune function, is used as a marker of the external defense function of zhengqi. ITRAQ and bioinformatics will be used to analyze the differential expression of proteins in the lung and descending colon of rats between the normal group, ADE group, NDE group and SP-D ko ADE group, and SP-D ko NDE group, so as to find and verify more valuable clues during comparing the micropathogenesis of autumn dryness evil and northwest northwest dryness group.This study provides a basis for the elucidation of "lung & large intestine interior-exterior relationship" theory and the molecular mechanism of external dryness syndrome based on the pathogenic factors of dryness, and also makes preparations for the exploration of TCM prevention and treatment of external dryness syndrome in arid and semi-arid areas based on the essence of the syndrome.