在应用各种四聚体分析结核标本时发现多肽/HLA-DR/CD4 TCR之间存在漂移结合现象，8种E7/HLA-DR四聚体分选出的细胞为相同或高度相似的CD4+ T细胞克隆。本项目拟以E7多肽相关的8种HLA-DR四聚体和3种CD4+ TCR四聚体-流式细胞以及E7/HLA-DR四聚体-ELISPOT进一步对同一结核患者标本作同步检测，用E7/HLA-DR四聚体分选患者CD4+ T细胞克隆进行结合-ELISPOT、增殖刺激与阻断试验，序列分析各种试验T细胞CDR3区及V-D-J及患者HLA-DR等位基因。期望能通过分析四聚体与结核患者的HLA-DRB等位基因对应与否，以及CD4+ T细胞克隆的TCR CDR3区和V-D-J 均一性与否，探讨HLA-DR等位基因、CD4+ T细胞克隆的TCR CDR3区与V-D-J 序列或空间构象对多肽/ HLA-DR / TCR之间结合识别的影响与规律。

Drift binding between Mycobacterium tuberculosis (MTB) peptide/HLA-DR―CD4 TCR was found during analyzing samples from patients with tuberculosis (TB) using peptide/HLA-DR tetramers or CD4+ TCR tetramers, and same or highly similar CD4+ T cell clones could be sorted using 8 kinds of peptide E7/HLA-DR tetramers. This program is intended to detect samples from more same TB patients using flow cytometric analyses with E7-related 8 kinds of peptide E7/HLA-DR tetramers and 3 kinds of CD4+ TCR tetramers and ELISPOT assay with 8 kinds of peptide E7/HLA-DR tetramers, monitor CD4+ T cell clones sorted by 8 kinds of peptide E7/HLA-DR tetramers by using binding-ELISPOT and proliferation stimulus and blocking test, and then sequence and analyze the sequences of CDR3 and V-D-J of T cell clones and the patients' HLA-DR allels. Hope by analyzing matched or unmatched HLA-DR allels between tetramer and patients and uniformity or heterogeneity of CDR3 region and V-D-J in CD4+ T cell clones, explore that HLA-DR allels, and sequences and spatial conformation of CDR3 and V-D-J influence on recognition and binding and their law between peptide/HLA-DR―CD4 TCR.