本课题组建立了原醛症的诊治流程及样本数据库，在既往实验的基础上，进一步研究发现YAP1基因在醛固酮瘤组织中高表达。YAP1是转录共激活因子，现认为YAP1（Yes-associated protein 1）可与β-catenin形成复合体参与肿瘤发生。本研究旨在探索YAP基因及Wnt/β-catenin通路在调控醛固酮的分泌与合成以及醛固酮瘤的发生中的作用；通过进一步建立肾上腺皮质条件性转基因小鼠模型，研究YAP1通过Wnt/β-catenin通路对于醛固酮瘤的增殖、凋亡以及分泌功能的影响，并通过小分子抑制剂干预这一通路来调节醛固酮的分泌。本研究还将进一步利用原醛症患者双侧肾上腺静脉采血标本及术前术后血标本检测cell-free DNA，为治疗提供新靶点及新思路，同时为实现基因分型、准确判断预后及预测复发提供新的生物标记物。

Our research group has established diagnosis-to-treatment procedure and tumor tissue bank of primary aldosteronism (PA). On the basis of novel findings of previous work, we found that YAP1(Yes-associated protein 1) was overexpressed in aldosterone producing adenoma(APA). YAP1 is a transcriptional co-activator, and it was reported that YAP1 and β-catenin could be compound and activated Wnt/β-catenin signaling pathway. We aim to explore the role of YAP1 and Wnt/β-catenin signaling pathway on aldosterone production in APA. Based constructing adrenal cortex selectively knock-out mice and regulating this process by small molecular inhibitor, we are going to conduct the research on YAP1 regulating aldosterone production by regulating Wnt/β-catenin signaling pathway. Furthermore, we will test cell-free DNA on the blood sample from adrenal venous sapling and patients’ follow-up after surgery treatment. This study aims to provide prospects of new treatment and biomarkers in gene classification, prognosis and recurrence of APA.