CTL2是AIED的目标抗原之一。利用杆状病毒作载体，将外源基因CTL2-P1和CTL2-P2转染sf9细胞后，通过Western Blot和免疫荧光法来检测CTL2-P1和CTL2-P2的表达，得到高纯度的重组CTL2亚型蛋白。用纯化CTL2亚型蛋白分别免疫小鼠，制备AIED动物模型。收集AIED病人及AIED动物血清， ELISA和Western Blot检测血清中重组CTL2抗体。通过以上研究阐明CTL2亚型在AIED中的作用，同时也为临床上诊断AIED提供一种重要的标志物。

CTL2 was discovered in the inner ear as a supporting cell antigen that is the target of antibody induced AIED .To study whether inner ear supporting cell antigen CTL2 can be cloned by recombinant baculovirus. Determine if mice immunized with CTL2 will induce AIED and if patients with AIED have antibodies to rCTL2. Mice inner ear CTL2 mRNA was cloned into baculovirus. The purified recombinant plasmid containing CTL2-P1 and CTL2-P2 gene was transfected into sf9 insect cells to express recombinant protein CTL2-P1 and CTL2-P2．The recombinant protein expressed was detected by Western blot and immunofluorescent assay．AIED patient sera were tested for antibodies to purified rCTL2 on western blots and ELISA. The study will elucidate the function of CTL2 isoforms in AIED and provide an important diagnostic marker to detect AIED.