母猪子宫内膜炎（SM）是规模化猪场常见的繁殖障碍性疾病，该病发病率高，治愈率低，已成为母猪非生理性淘汰的主要原因。最新的研究表明辅助性T细胞(Th)各亚群（Th1/Th2/Th17/Tregs）的免疫平衡在免疫系统的精细网络调控中发挥重要作用，其对机体局部或全身炎症反应的发生和转归产生直接影响。对于SM感染过程中Th细胞各亚群的免疫动态平衡状态及其调控机制还未有报道。本研究将建立SM模型，采用流式细胞术、ELISA方法研究患病母猪不同时相Th细胞各亚群及相关细胞因子的免疫平衡状态变化规律；采用基因芯片技术来揭示子宫壁局部免疫平衡相关基因表达谱的差异，并采用免疫组化和定量PCR等方法对差异表达基因进行验证；初步开展CD4+T各亚群及其相关差异表达基因的调控研究，探究其对SM感染的影响。本研究首次从免疫平衡角度来系统的探究SM感染过程中的免疫网络的调控机制，其结果将为科学防治SM提供理论支撑。

Sows endometritis is a common reproductive disorder disease in large-scale pig farms. It is the main cause to cull sows non-physiologically due to high incidence rate and low cure rate. The latest research shows that the immune balance of the T helper cell (Th cell) subsets (Th1/Th2/Th17/Tregs) plays an important role in regulation of the immune system, which has a direct effect on the occurrence and outcome in local or systemic inflammation. There are no reports regarding the immune dynamic balance state of the Th cell subsets in SM.In this research, a SM model will be developed and flow cytometry and ELISA methods will be used to study the rules of the immune balance of Th cell subsets and their related cell factor in different time points; Gene chip technology will be performed to obtain the profiling of differentially expressed genes in uterine wall; To validate the differentially expressed genes related the immune balance, real-time PCR and immunohistochemisty will be used , respectively.The regulation pathyway of these differentially expressed genes and subsets of CD4+T cells will be studied preliminarily. This research will firstly explore the accurate regulation mechanism of the immune system in SM from immune balance. These results will provide theoretical support for the scientific control of SM.