基于IFITM1/Wnt/β-catenin信号通路调控骨肉瘤细胞恶性增殖探讨抑瘤 汤抗骨肉瘤的机制

骨肉瘤(OS)是青少年易发生的恶性肿瘤之一，其发生和发展的特点是肿瘤恶性增殖和转移，目前缺乏有效的防治措施。抑瘤汤在临床中治疗OS患者，展现良好的效果，但是其作用机理还不清楚。申请者在前期工作中发现IFITM1在瘤体中高表达，经IFITM1 RNAi慢病毒转染U2OS后细胞增殖、迁移降低，凋亡增多。经查阅资料后证实，IFITM1在多种肿瘤的发生、发展中发挥重要的作用，其表达可能是通过Wnt/β-catenin信号通路调控。而Wnt/β-catenin信号通路的激活恰恰和肿瘤的增殖密切相关。故我们推测Wnt/β-catenin通路的激活对于OS的恶性增殖至关重要。本项目拟在此基础上，从大样本回顾性分析中总结IFITM1对于OS的发生、外科分级、预后等的相关性。同时，在细胞和动物模型中进一步探讨IFITM1/Wnt/β-catenin调控OS恶性增殖及抑瘤汤抗OS作用的分子机制。

Osteosarcoma (OS) is one of the malignant tumors which most likely to occur in adolescents. The Characteristics of occurrence and development is malignant proliferation and metastasis.At present, there is a lack of effective treatment measures.Yiliu soup Shows good results in clinical.But its mechanism is not clear.In the previous work, the IFITM1 was found to have high expression in the OS.After transfect U2OS with RNAi IFITM1 lentivirus,cell proliferation and migration decreased and apoptosis increased.Confirmed by the access to.information, IFITM1 play an important role in the occurrence and development of all kinds tumors.Its expression may be regulated by the Wnt/β-catenin signaling pathway.The activation of Wnt/β-catenin signaling pathway is closely related to the proliferation of tumors.So we hypothesized that activation of the Wnt/β-catenin pathway is essential for the malignant proliferation of OS.On the basis of the above,we hope to summarize the correlation of IFITM1 with the tumor occurrence, surgical classification and prognosis by the retrospective analysis of large samples.At the same time, Further explore the molecular mechanism of IFITM1/Wnt/β-catnin pathway regulation OS malignant proliferation and Yiliu soup treatment OS in the cell and animal model.