毛刺科线虫（Trichodoridae）是一类重要的植物外寄生线虫，目前已描述种超过90种。该类群内有些种类及群体除本身可寄生植物危害外，更重要的一个特征是其系一些植物病毒的自然传播介体，在相当长的一个阶段一直是国际植物检疫界关注的一个重要的线虫类群。本申请项目结合本实验室前期的研究基础，拟通过对我国不同地理生态地区（毛刺类线虫的主要栖息地）毛刺类线虫的主要地理种群的形态学、核糖体RNA基因中编码区和非编码区、Hsp90基因等的克隆及序列分析，明确我国毛刺类线虫的主要种群、种间及种内的形态学变异、核糖体RNA基因和Hsp90基因等的主要特性及其系统学关系、筛选一些重要种鉴定特征的分子靶标和建立针对一些重要种群的标准化分子诊断系统。研究结果将填补国际空白，对该类线虫的分子分类、有害生物风险评估及出入境检验检验部门和农林业生产部门针对性的采取相应的预警和检疫有重要的理论意义和直接的应用价值。

Nematodes in Family Trichodoridae constitute a gorup of important ecto-paraistes of plant. Until now more than 90 species have been described and reported in the group. Except for as parasites of plant, the other important character of the group of nematode is that many species and populaitons in the group can be the plant virus vectors, and the nematodes in the Family are world concerned.In this study the species and populations in the group will be sampled from different bio-types in China. Morphological and molecular identification, including rDNA and Hsp90 gene, will be made. To characterize the major trichodorid species occurring in China, morphological variations of inter- and intro-species populations, rDNA and Hsp90 gene characters,and the phylogeny relation of the species will be studied. Based on screening the speices specific molecular markers, a stand species-specific molecular identification method for the important species will be constrcuted. The research results will provided some new data, and it will be very helpful and usful for molecular identification and taxonomy, pest risk analysis, and proper intergreted management methods to the Plant quarantine, agriculture and forestry departments in China.