本课题选择Ru(II)配合物光诱导抗癌活性作为治疗方法，细胞荧光学成像技术作为诊断方法并辅以电镜等观测。其中光化学疗法是一种新型的肿瘤治疗技术，在对光诱导Ru金属配合物切割DNA的活性考察中，发现其在光诱导下可以有效地抑制肿瘤细胞活性，表现出令人鼓舞的应用前景。而细胞荧光成像不但有低的信号背景能高效的观测细胞层次的变化而且成本较低，操作简便，检测快速。本项目的特色和创新之处在于，将光化学治疗与细胞荧光成像相结合。基于此以金属基Ru配合物的荧光性质及光致DNA切割为前期基础研究，进而把研究扩展到金属Ru(II)配合物的设计、合成，及它们在可见、近红外可见光诱导下对肿瘤细胞的作用和肿瘤细胞形态的变化，实现在观测中治疗和在治疗中检测的双重目的。

In this study, we choose photo-induced anticancer Ruthenium(II) complexes as treatment reagent and fluorescent probe in cell as diagnosis reagent, accompanied by SEM study. photo-induced anticancer is a novel valuable method for the treatment of cancer. In the study of photo-induced DNA cleavage, Ru(II) complexes inhibits tumor cell activity effectively, showing encouraging application prospect. Confocal microscope has advantages of lower background signal, efficient change measurements at cellular level, low cost, simple operation and fast detection. This proposal presents the novel integration of photo-induced anticancer and fluorescent probe in cell. Based on the prevenient research of fluorescence properties and photo-induced DNA cleavage of metal Ru(II) complexes, theranostic reagents Ru(II) complexes are designed and synthesized accompanied with the investigation of their impact on tumor cells and morphological changes of the cancer cells, realizing double-edged mission of detection of tumor and targeted therapy.