V-ATPase由V1和V0两个结构域构成，V1催化结构域由8个亚基（A，B，C，D，E，F，G，H）构成，V1作为催化结构域对昆虫维持细胞PH值有重要作用。课题申请人已克隆出棉铃虫V-ATPase V1结构域A、B、C和H亚基。本项拟克隆V1结构域D，E，F，G亚基，研究V1结构域8个亚基在棉铃虫虫不同龄期不同组织的表达量，建立RNA干扰体系，研究不同亚基对棉铃虫V-ATPase酶活、ATP含量、体重、体长、存活率和蜕皮的影响，在此基础上分析8个亚基的功能与作用。对棉铃虫V-ATPase V1 结构域进行研究，有利于掌握鳞翅目害虫的V-APTase的结构和功能，为鳞翅目害虫的生物技术防控提供理论依据。

V-ATPase is composed of V1 and V0 domains.Domains V1 is composed of 8 subunits (A, B, C, D, E, F, G, H) , which have an important role as the catalytic domain to maintain cell pH on insects. A, B, C and H subunits of Cotton bollworm V-ATPase V1 had been cloned. This project Would clone D, E, F, G subunit of cotton bollworm and the mRNA expression of 8 subunits would been measured by fluorescence quantitative PCR in different tissues and developmental duration of cotton bollworm .Then RNAi system would been.established and to Study on effect of V-ATPase enzyme activity, ATP content, body weight, body length, survival rate and molting by RNAi of different subunits of cotton bollworm V-ATPase V1.To study the eight submits of cotton bollworm V-ATPase V1, is conducive to grasp the structure and function of lepidoptera pests of V-APTase and provide theoretical basis for lepidoptera pests prevention and control of biological technology.