类风湿关节炎（RA）早期即存在滑膜异常代谢、血管新生，最终致骨破坏，呈动态的相关变化,血管新生是其中重要的中间过程。血管新生与中医络病相关。前期工作表明，以温经通络法治疗RA，对血管生成因子有干预作用。基于前期工作，本研究开展以下工作：（1）体内研究：复制胶原诱导关节炎（CIA）大鼠模型，在病变早期应用磁共振波谱及成像方法，从能量代谢及影像学角度动态观察类风湿关节炎滑膜血管新生及软骨破坏的动态变化，并用分子生物学手段，分析相关细胞因子及信号通路的动态改变；基于温经通络法，以芍药甘草附子汤加味为干预手段，研究其调控RA滑膜血管新生，从而抑制软骨破坏的生物学依据；（2）体外研究：CIA大鼠关节成纤维样滑膜细胞为研究对象，以中药血清学方法，研究温经通络法对血管新生相关因子的调控作用。目的 从RA早期病理生理变化-血管新生为切入点，研究温经通络法调控滑膜血管新生从而抑制软骨破坏作用。

There are the synovial abnormal metabolism,angiogenesis in rheumatoid arthritis(RA),which will lead to bone destruction ultimately,and angiogenesis is the important intermediate process.Angeogenesis is related with collateral disease of chinese medicine.Preliminary work showed that Tongluozhibi method had effects on the angilgenic factors.In this study，these works will be doned as followed (1) duplicating the collagen-induced arthritis(CIA) rat model, magnetic resonance imaging(MRI) and magnetic resonance spectroscopy (MRS) willed be used to observe the changes of angiogenesis and bone destruction dynamically from the points of energy metabolism and imaging destuction;The molecular biology methods be used to study the relevant cytokines and signaling pathways changes；Meanwhile ,Wenjingtongluo method will be used to study the regulating effects on the synovial angiogenesis and cartilage destruction to find the biological effects of evidence; (2) the rat joints fibroblast-like synovial cell of collagen-induced arthritis will be uesd in vitro to study the regulating effects of Wenjingtongluo Method on the angiogenesis fators. Purpose to find the regulating effects of Wenjingtongluo method on RA from the points of angiogenesis and cartilage destruction.