DNA损伤修复在基因组维护与肿瘤发生发展及诊治中都发挥了至关重要的作用。蛋白相互作用是蛋白实现它们各自功能的基础。系统地了解体内蛋白复合物组成及复合物间的相互作用，可以极大地促进对细胞生命活动机理的探索。然而由于高通量的蛋白复合物纯化技术的限制，系统的蛋白相互作用组的研究还进行得非常少，包括DNA修复领域。我们发展了一套适用于高通量的蛋白复合物纯化技术。应用这项技术，我们将对DNA损伤修复进行相互作用组的研究。在前期工作中，我们已经完成了263个DNA修复相关的蛋白复合物（>85%已知DNA修复蛋白）的纯化和质谱鉴定工作，并着重对双链断裂修复新蛋白进行了功能和机制研究。我们将继续这项相互作用组研究，用于建立一个相对完整的DNA修复相关的蛋白相互作用网络图谱，并着重研究双链断裂修复。该图谱的完成，将推动对DNA损伤修复在基因组维护中的作用机理的理解，从而促进肿瘤的防治和抗肿瘤新药的研制。

DNA repair is the core system for genome stability maintenance, which is highly related to cancer development and therapy. Protein interactions form the molecular backbone of cell biology, where proteins assemble into metastable complexes to constitute bioactive units. These complexes then dynamically interact with each other in context of larger networks to carry out varying biological functions. Thus, the interactome analysis of protein complexes and protein interaction networks is critical for biological research. However, limitations of high-throughput methods for protein complex purification hinder the interactome analysis, especially in human cells. We have developed a high-throughput method for measuring protein-protein interactions by affinity purification of tagged protein baits followed by mass spectrometry (AP/MS). With this method, we have purified 263 DNA repair related and 42 un-related protein complexes, which were identified by followed mass spectrometry. We will continue this work until a map of DNA repair protein interaction network is established. This map will provide a new insight of the genome stability maintenance mechanism, which will further promote the understanding of cancer development and cancer therapy.