系统性红斑狼疮（SLE）患病率约为70/10万，是严重危害人民健康的难治病，西医主要应用糖皮质激素（GC）等治疗，但存在副作用较多的困境。我们长期研究发现，狼疮定对GC治疗SLE具有较好的增效作用。.本项目拟通过阐明狼疮定对GC不同敏感性SLE pDC、T细胞GRα、GRβ分布、表达和核转位的调控作用；通过流式细胞术检测FITC-Dex与GRα的亲和力、构建GC应答元件（GRE）基因与萤火虫荧光素酶（luc）报告基因质粒检测转录活性、免疫共沉淀法检测GRα与NF-кB p65相互作用，阐明狼疮定对GRα与GC亲和力、转录活性及GRα与NF-кB p65互作的调控作用；通过筛选与GRβ互作蛋白，阐明狼疮定对GRβ及互作蛋白的调控作用，以明确狼疮定的增效机制，为中医药治疗SLE奠定科学依据，并提供药物作用靶点。

Being a refractory illness, the prevalence of systemic lupus erythematosus (SLE) is about 70 per 100 thousand, which seriously endangers people's health. Corticosteroids (GC) is the commonly used drug in treating SLE in Western medicine, but a lot of side-effects usually put doctors into a dilemma. In the long term study, we found that Lang-chuang-ding combined with GC has good effects in treating SLE. .In this study, we wish to clarify the regulating effects of Lang-chuang-ding on the distribution ,expression and nuclear translocation of GRα and GRβ in pDC and T cell of GC-sensitive or GC-insensitive SLE patients, and to detect the affinity of FITC-Dex and GRα by flow cytometry, and to detect the transcriptional activity by constructing restructuring plasmid with GC response element (GRE) genes and firefly luciferase (luc) reported gene plasmid, and to detect the test interactions of GR α and NF-к B p65 by immune total precipitation,therefore expound the regulation function of Lang-chuang-ding on affinity and transcription activity of GRα and GC and interaction of GRα and NF-к B p65,and to screening proteins interacted to GRβ, to clarify the regulation function of Lang-chuang-ding on GRβ and the interacted protein,so to analyze the synergism mechanism of Lang-chuang-ding for lupus,which may also to provide scientific data for traditional Chinese medicine treatment for SLE and provide drug targets.