马铃薯Y病毒（Potato Virus Y，PVY）严重影响辣椒(Capsicum spp.) 生产。在辣椒中pvr2-eIF4E基因编码翻译起始因子eIF4E，介导辣椒对PVY的抗性，是较稳定有效的抗源基因，具有序列多样性和抗性差异。本项目拟研究我国蔬菜种质资源中期库中1904份辣椒材料pvr2-eIF4E基因的多态性，据此构建专项核心种质，并对核心种质进行PVY抗性鉴定，分析pvr2-eIF4E基因与PVY抗性的关系，筛选PVY抗源材料；采用VIGS和CRISPR/Cas9转基因方法，进一步验证pvr2-eIF4E基因与PVY抗性的关系；采用酵母双杂交（Y2H）和双分子荧光互补（BiFC）的方法研究PVR2-eIF4E和PVY病毒基因组连接蛋白VPg的互作关系，结合生物信息学分析，揭示与PVY抗性相关的pvr2-eIF4E基因的关键变异位点，揭示其抗性作用机理。

The production of pepper (Capsicum spp.) could be reduced seriously by potato virus Y. In pepper, pvr2-eIF4E encodes eukaryotic translation initiation factor (eIF4E), which mediates PVY resistance effectively and durably. Pvr2-eIF4E is polymorphic in genotype and variable in PVY resistance. The project aims to examine the pattern of polymorphism of the pvr2-eIF4E coding sequence among 1904 pepper accessions from Chinese vegetable germplasm middle-term storage, and establish pvr2-eIF4E core germplasm. And then the pvr2-eIF4E core germplasm is going to be used for identification of resistance spectrum by artificial inoculation with PVY, thus verify the resistant pvr2-eIF4E genotypes and accessions. VIGS and transgenic analysis based on CRISPR/Cas9 are going to be used for pvr2-eIF4E gene function analysis, verifying the correlation between pvr2-eIF4E and PVY resistance. Y2H and BiFC are going to be used for interaction analysis between PVR2-eIF4E and VPg of PVY, and the important sites of pvr2-eIF4E for PVY resistance is going to be predicted combining with bioinformatic analyses, and thus reveal the resistant mechanism of pvr2-eIF4E.