K+通道在牵张力和孕酮协同调节分娩期大鼠子宫平滑肌舒缩时的作用研究

产科出血、早产分别是导致孕产妇及新生儿死亡的第一位原因。而分娩期子宫平滑肌舒缩功能与产科出血、早产的病因均密切相关。近期本课题组及相关研究发现，牵张力在分娩期可能与孕酮协同调节子宫平滑肌舒缩，而平滑肌中的特异性钾离子通道可能在此协同调节过程中起关键调控作用。但是，目前对牵张力及孕酮的协同作用特点、以及钾离子通道在此过程中的具体作用均不明确。本项目拟使用分娩期大鼠子宫平滑肌为模型，通过离体子宫平滑肌的舒缩功能变化特点，研究牵张力及孕酮在调节子宫平滑肌舒缩时的协同作用；同时通过子宫平滑肌生理功能变化、K+通道标记分子基因及蛋白表达水平检测等方法，研究不同K+通道在牵张力及孕酮协同刺激子宫平滑肌时的作用特点；从而明确调节妊娠子宫平滑肌舒缩时的关键调控因子，最终为临床人为调控妊娠子宫平滑肌舒缩提供可能的解决途径。

Obstetric hemorrhage, premature birth were the first reason lead to maternal and neonatal death respectively. Function of term myometrium contraction is closely related to obstetric hemorrhage and premature delivery. Recently we found that stretch may function with progesterone coordinately during myometrium contaction. Specific potassium channels may regulate the process of myometrium contraction under stretch and progesterone stimulation. Until now, we were not sure about characteristics of stretch and progesterone colaboration, and function of potassium channels in this process. This project intends to use term rat uterine smooth muscle as model to study the characteristics of in vitro myometrium contraction variation when stimulated with stretch and progesterone. At the same time we will study the function of different potassium channels in term myometrium under stretch and progesterone, followed with the protein expression of different potassium channels. Finally we would find the key protein in the process of term myometrium contraction under stretch and progesterone stimulation.The findings will give great help to clinical intervention with uterine smooth muscle contraction during delivery.