SpoT在幽门螺杆菌生物膜形成中的调控机制研究

生物膜是幽门螺杆菌（H.pylori）生存及传播过程中的一种重要适应性生存方式，目前关于H.pylori生物膜的形成机制仍知之甚少。我们的前期工作证实血清饥饿可诱导H.pylori生物膜的形成，SpoT参与多种细菌生物膜的形成，并有研究报道SpoT在H.pylori应对血清饥饿严谨应答中起重要作用，但其具体作用机制不明确，由此我们推测SpoT在H.pylori生物膜形成中发挥调控作用。因此，我们构建了SpoT基因缺失突变株，拟利用蛋白质组学筛选H.pylori野生株和SpoT突变株形成生物膜后的差异蛋白质，寻找生物膜形成过程中受SpoT调控的蛋白质分子，并在分子水平和动物水平对筛选出的关键蛋白进行体内外功能验证，分析SpoT在H.pylori生物膜形成中的调控机制，将有助于揭示H.pylori抗逆生存机制和发现新的致病因子及抗菌靶位，旨在为H.pylori的预防与治疗提供新思路。

Biofilm is one of important adaptive living way of Helicobacter pylori ( H.pylori ) during its survival and propagation. However, the formation mechanism of H.pylori biofilm is still poorly understood. Our previous work has confirmed that serum starvation can induce biofilm formation of H.pylori. SpoT is involved in biofilm formation of a variety of bacteria and has been found play an important role in the strigent response of H.pylori to serum starvation. But the concrete regulatory mechanism is not clear. Thus we associate that SpoT may contribute to the regulation of H.pylori biofilm formation. Based on the above analysis, we constructed the SpoT mutant strain of H.pylori. In order to find proteins regulated by SpoT in the biofilm formation of H.pylori under serum starvation, comparative proteome analysis will be used to compare the proteomic profile of the biofilm in the wild-type H.pylori and the SpoT mutant. The function of key proteins discovered will be identified on molecular and animal level. This work will be useful for the identification of new pathogenic factors and curative value against H.pylori and will provide new thinking for the prevention and treatment of this pathogenic bacteria.