水稻边界发育缺陷突变体abnormal boundary development(abd)的基因克隆与功能分析

植物一生中不断产生新器官，新器官的形成会伴随边界区域的产生。边界区域发育不仅是一个关键的生物学问题，同时也是一个与产量息息相关的重要农业性状。在单子叶植物水稻中，边界区域可以划分为三类：器官-分生组织边界，内环边界和组织内边界。叶枕区发育属于组织内边界发育。通过突变体筛选，获得了一株水稻叶枕发育缺陷突变体abnormal boundary development/abd），图位克隆及互补实验表明，ABD编码一个NAC类转录因子，是拟南芥CUC1 CUC2基因的同源基因。本研究拟以abd突变体为材料，重点解析abd在水稻不同边界区域，尤其是叶枕区的功能，并分析生长素和细胞分裂素在水稻边界区域发育过程中的调控作用；深入探讨abd及不同激素可能对水稻边界区域建立的作用方式，从而为理解边界区域发育，改变植物株型，提高作物产量奠定理论基础。

In plants, new organs are formed during the whole life cycle. New organ formation will lead to boundary formation. Boundary development is not only an essential biological issue, but it is also a very important agronomic trait which strongly influences crop yield. In monocot crop rice, boundaries can be defined as three groups: Organ-meristem boundaries, intra-whorl boundaries and within-organ boundaries. Leaf collar aera belongs to within-organ boundaries. One collar development defect mutant named as abnormal boundary development(abd) was obtained after screening of rice mutants. Via map-based cloning and complementation experiment, we found ABD encodes a NAC transcription factor which is the ortholog of Arabidopsis CUC1 and CUC2 genes. We will analyze the abd mutant and try to investigate the role of ABD in rice boundaries development, especially in leaf collar development. Furthermore, we will focus on the function of auxin and CK in the process of boundary formation. In addition, we will investigate the mechanism how abd, auxin and CK modulate rice boundary development. This work will shed more light on understanding boundary formation as well as plant architecture, and provide genetic resources to improve crop yield.