胰岛β细胞功能障碍和数量减少是糖尿病发生的关键因素。胰岛素在胰岛β细胞粗面内质网合成修饰后转运至高尔基体并最终释放入血，内质网至高尔基体转运是胰岛素合成分泌的重要过程，转运障碍导致的内质网应激是胰岛β细胞功能障碍及凋亡的重要原因。cTAGE5/TANGO1在胰岛素由内质网至高尔基体的转运中发挥重要作用。我们前期研究发现健脾消渴方可改善胰岛素分泌，促进胰岛β细胞自噬，抑制凋亡。为深入探讨其机制，本研究拟体内体外研究结合，体内通过敲低大鼠cTAGE5/TANGO1的表达，观察健脾消渴方对大鼠血糖、胰岛素分泌、胰岛细胞数量与分布、自噬及cTAGE5/TANGO1表达的影响；体外通过敲低min6细胞系cTAGE5/TANGO1表达，观察健脾消渴方对胰岛素分泌，内质网应激，细胞自噬、凋亡及cTAGE5/TANGO1表达的影响。从分子层面探讨健脾消渴方调节胰岛β细胞机理，为中医药防治糖尿病提供依据。

Islet β-cell dysfunction and cell number reduction are key factors in the development of diabetes. Insulin is synthesized and modified in islet β cell rough endoplasmic reticulum and then transport to the Golgi apparatus and eventually released into the blood，endoplasmic reticulum to Golgi transport process is an important process of insulin synthesis and secretion, endoplasmic reticulum stress caused by transport disorders is an important cause of pancreatic β-cell dysfunction and apoptosis. cTAGE5 / TANGO1 plays an important role in the insulin transport from the endoplasmic reticulum to the Golgi apparatus. Our previous study found that Jianpi Xiaoke Decoction can improve insulin secretion,promote islet β-cell autophagy, inhibit pancreatic β-cell apoptosis. In order to further explore its molecular mechanism, this study intends to combine the in vivo and in vitro studies, by knocking down rat cTAGE5 / TANGO1 expression in vivo to observe the effects of Jianpi Xiaoke Decoction on blood glucose, insulin secretion, islet cell number , autophagy and expression of cTAGE5 / TANGO1 in vitro. The effect of Jianpi Xiaoke Decoction on insulin secretion, endoplasmic reticulum stress, autophagy, apoptosis and the expression of cTAGE5 / TANGO1 was observed in vitro by knockdown of cTAGE5 / TANGO1 in min6 cell line. To further explore the mechanism of Jianpi Xiaoke Decoction on the function of pancreatic β-cells from the perspective of molecular cell, and provide evidence for the prevention and treatment of diabetes by traditional Chinese medicine.