胚胎植入是妊娠过程的关键环节，其发生的好坏直接决定了妊娠的最终结局。子宫腔上皮作为与胚胎接触的直接位点，其结构和功能的完整性对于胚胎植入的发生具有重要作用。子宫接受态建立过程中，子宫上皮细胞不断转化，发生去极化和分化等改变。申请人在前期的研究中发现，子宫特异性敲除Rac1分子后导致子宫上皮细胞转化异常，进而影响胚胎植入过程，其中Ezrin信号通路明显受到抑制。本研究中，利用敲除小鼠模型及体外模型，结合生化、分子、细胞等技术，进一步探究：（1）Ezrin在胚胎植入过程中的动态表达及定位模式。（2）Ezrin介导的子宫上皮细胞转化在胚胎植入过程中的具体生理作用。（3）Ezrin在人子宫内膜接受态建立过程中的调节机制。（4）Ezrin参与调节子宫上皮细胞转化的潜在分子机制。本研究将进一步加深对胚胎植入过程调节机制的了解，为治疗胚胎植入缺陷导致的妊娠失败提供新的理论基础和新思路。

Embryo implantation is one of the key steps during pregnancy and the quality of this process determines the result of pregnancy. As the direct site of embryo attachment in uterus, the integrity of the epithelial structure and function is essential for the embryo implantation process. In our previous finding, uterine loss of Rac1 leads to defects in uterine epithelial transformation, which cause the failure of embryo implantation. Further detailed analysis showed that the activation of Ezrin signaling is severely affected. In the current study, using the conditional knockout mouse model and in vitro cell model, combined with immunofluorescence, immunoblotting and electron microscopy methods, we will further study: (1) the dynamic expression pattern of Ezrin in the periimplantation uterus. (2) the physical role of uterine Ezrin in embryo implantation process. (3) the function of Ezrin in human endometrial receptivity establishment. (4) the molecular mechanisms of Ezrin in regulating the uterine epithelial cell transformation. It is hoped that a better understanding of the embryo implantation process will provide new guidelines for treating the pregnancy failure led by the embryo implantation defects.