低场核磁共振均相免疫分析及其海洋病原体高灵敏现场检测应用研究

海洋病原体的高灵敏、准确、简单、快速现场检测有重要意义。传统方法各具优势但不适用于现场检测，光电生物传感器法也存在两个问题：非均相免疫分析固有的多步操作、信号产生受到样品基底的严重干扰。本项目设计制备多功能化超顺磁二维材料，既是低场核磁共振的磁信号探针也是均相免疫分析的捕获单元，创立低场核磁共振均相免疫分析，兼具低场核磁共振不受样品基底干扰和均相免疫分析一步完成之优点，解决上述问题。并且，多功能化超顺磁二维材料对低场核磁共振信号有三级增强：磁矩更大、影响更多水分子、协同作用增强，显著提高灵敏度。基于外差式锁相放大技术对微弱的低场核磁共振信号的识别、提取和放大，研制低场核磁共振便携式现场检测装置，结合多功能化超顺磁二维材料，构建高灵敏快速现场检测平台，一个装置、一个探针、一步完成，实现海洋病原体的高灵敏、高选择、免干扰、准确、简单、快速、价廉的现场检测，具有重要科学意义、社会效益和应用前景。

Highly sensitive, accurate, simple and fast field detection of marine pathogens is of great significance. Traditional methods have their own advantages, but they are not suitable for field detection. Optical and electrochemical biosensors also have two problems: the multi-step operation in heterogeneous immunoassay is inherent, and the signal generation is severely disturbed by the sample substrate. In this project, the multi-functional superparamagnetic two-dimensional material is designed and prepared, which is not only the magnetic signal probe of low-field nuclear magnetic resonance (NMR), but also the capture unit of homogeneous immunoassay. Based on it, low-field NMR homogeneous immunoassay is created, which combines the advantages of low-field NMR which is unaffected by sample substrate and homogeneous immunoassay which could be accomplished in one step. Thus, above two problems could be solved. In addition, the multi-functional superparamagnetic two-dimensional material has three-level enhancement of low-field NMR signals: possessing larger magnetic moments, affecting more water molecules and enhancing the synergy, so the detection sensitivity could be significantly improved. Through the identification, extraction and amplification of weak low-field NMR signals by heterodyne lock-in amplification technology, a low-field NMR portable field detection device is developed. Combining the device with the multifunctional superparamagnetic two-dimensional material, a highly sensitive and fast field detection platform is constructed. Highly sensitive, highly selective, interference-free, accurate, simple, fast and cheap field detection of marine pathogens can be obtained using only ONE device, ONE probe and ONE step. It has important scientific significance, social benefits and application prospects.