神经分化率低下是骨髓间充质干细胞(BMSCs)移植治疗脊髓损伤(SCI)面临的重要问题，因此，提高BMSCs定向神经分化，对SCI修复至关重要。既往研究已证实MAPK/JNK是BMSCs促神经分化经典信号通路，而Notch是抑制信号通路。近期我们预实验发现Wnt5a具有激活MAPK/JNK和抑制Notch信号通路的双重作用，过表达Wnt5a的BMSCs具有高效的神经分化能力；而补肾活血方可显著上调Wnt5a表达。由此我们提出假说：Wnt5a通过激活MAPK/JNK通路、同时抑制Notch信号通路，获得高效BMSCs定向神经分化趋势；另一方面补肾活血方通过体内上调Wnt5a表达，进一步提高BMSCs神经分化率，进而恢复神经功能，重建神经回路，达到修复SCI目的。本课题采用体外-体内、分子-细胞-整体水平，从基因表达、组织学及动物形态学三个层次证明该假设，为高效治疗SCI提供新靶标和思路。

BMSCs transplantation has always been faced with the problem of low neuron differentiation rate in vivo. Therefore, improving the directional neuronal differentiation rate of BMSCs play an important role in the treatment with SCI. Previous studies reported that MAPK/JNK was a classic signaling pathway for BMSCs to promote neural differentiation, and Notch was a signaling pathway for BMSCs to inhibit neural differentiation. Recently, our results showed that Wnt5a had the dual effects of activating the MAPK/JNK pathway and inhibiting the Notch signaling pathway. Furthermore, We also found that a stronger ability was measured under the Wnt5a-overexpressed BMSCs to direct neural differentiation, and Bushen Huoxue decoction can significantly increase Wnt5a expression. Therefore, we hypothesize that Wnt5a-overexpressed BMSCs has a better ability to direct neuronal differentiation by activating the MAPK/JNK signaling pathway and inhibiting the Notch signaling pathway. After transplanting Wnt5a-overexpressed BMSCs into spinal cord injury and combining with Bushen Huoxue decoction, a higher rate of neural differentiation can be obtained, which can restore nerve function, rebuild neural circuit and improve the motor function in spinal cord injury. To prove this hypothesis, we use the different the biological technology, including the vitro-vivo, molecular-cell-integral level, gene expression, histology and animal morphology. The potential findings may provide new targets and ideas for accurate treatment with spinal cord injury.