SOX4调节PR泛素化修饰在胚胎植入和妊娠维持中的作用机制

蜕膜正常发育是决定妊娠成功与否的关键环节。孕酮受体PR是子宫内膜蜕膜化的过程中一个关键的分子，PR受体缺陷影响妊娠的发生和维持，目前关于PR受体缺陷机制还知之甚少。我们前期研究发现SOX4可能是影响PR受体缺陷的原因，本项目拟进一步通过人子宫内膜基质细胞体外诱导分化体系，和结合临床使用蜕膜化障碍子宫内膜标本，利用现有的分子生物学技术手段将阐明SOX4和PR在胚胎种植及妊娠维持过程中的作用，深入研究SOX4调节PR蛋白泛素化修饰在子宫蜕膜化过程中的具体机制，拓宽我们对胚胎植入和妊娠维持相关机制的认识，为反复种植失败和复发性自然流产患者的诊断和治疗提供实验基础。

The normal development of decidua is the key to the success of pregnancy. Progesterone receptor PR is a key molecule in the decidualization of endometrium. PR receptor deficiency affects the occurrence and maintenance of pregnancy. At present, little is known about the mechanism of PR receptor defects. Our previous studies have found that SOX4 may be the cause of PR receptor defects. This project intends to further clarify the role of SOX4 and PR in embryo implantation and pregnancy maintenance through an in vitro induced differentiation system of human endometrial stromal cells and using decidualization disorder endometrium specimens. To further study the specific mechanism of SOX4 regulating the ubiquitination modification of PR protein in decidualization of uterus. Broaden our understanding of the mechanism of embryo implantation and pregnancy maintenance, and provide experimental basis for diagnosis and treatment of patients with recurrent implant failure and recurrent spontaneous abortion.