天然免疫识别由识别病原相关分子模式(PAMP)的模式识别受体(PRR)介导。甘露聚糖结合凝集素（MBL）和Toll样受体 (TLR) 均是参与天然免疫识别的重要PRR。本课题组的最新研究发现MBL参与调节LPS-TLR4通路从而抑制树突状细胞的免疫功能、MBL可与TLR4直接结合且MBL的糖识别域（CRD）可识别TLR配体。据此提出本项目，拟就MBL与TLR之间的相互作用进行下述研究：①MBL与TLR及TLR配体的结合特性；②MBL对TLR信号转导通路的调节；③MBL对TLR配体诱导免疫应答及炎症反应的调节作用。其创新点和意义为：①揭示MBL与TLR及TLR配体的相互作用及其特性，将加深了解天然免疫识别的机理；②阐明MBL对TLR通路调节作用的机制，提出MBL调控TLR介导免疫应答和炎症反应的新观点，将为人工调控有关炎症性疾病提供新的理论依据。

Innate immune recognition relies on a limited numbers of pattern recognition receptor (PRR) respond to pathogen-associated molecular pattern (PAMP). Toll like receptor (TLR) and mannan-binding lectin (MBL), two major PRRs, play crucial role in the induction of innate immune response. Our group have recently reported that MBL could regulate the activation of LPS-TLR4 pathway then inhibited the immune fuction of dendritic cell (DC). In addition, we found that MBL could directly interact with TLR4 and the carbohydrate recognition domain (CRD) of MBL bound to TLR ligands specifically. Here, we will extend our study to the relation of MBL and TLRs. First, we will determine the binding characteristics of MBL to TLRs. Secondly, the immunoregulatory role of MBL in TLR pathway will be studied, including the cytokine production by DCs and the antigen presenting capacity of DCs upon TLR ligands stimulation. Furthermore, the regualtion of MBL on TLR mediated immune response and inflammation will be studied. This research will enable us to have a better understanding of the mechanism for innate immune recognition and MBL regulate the TLR mediated innate immune responses and inflammation. The results will provide a theoretical basis for the therapies of infectious diseases.