青光安Ⅱ号方对慢性高眼压大鼠视网膜神经节细胞压力敏感离子通道受体蛋白的影响研究

青光眼是一种不可逆的视神经病变，在全球范围内是引起失明的第二大原因。高眼压是青光眼疾病的主要危险因素，可引起视神经损伤，对于该种损伤国内外尚无有效治疗方法，亟需有效治疗药物出现。中医药在该领域拥有广阔的前景，有发现新复方的巨大潜力。中医认为该视神经损伤的病机在于肝肾亏虚，气虚血瘀。基于滋补肝肾，益气活血的治法，我们创制了青光安Ⅱ号方。前期实验研究已证实，青光安Ⅱ号方对慢性高眼压视神经损伤有保护作用，但其具体作用机制尚不明确。有研究显示视网膜神经节细胞膜表面有机械应力感受受体及作为相应的机械应力感受器及效应器的离子通道受体与视网膜神经节细胞凋亡有关。本研究观察该方对慢性高眼压大鼠视网膜神经节细胞压力敏感离子通道受体的影响，探讨其抗慢性高眼压视神经损伤的分子细胞学机制，为中医治疗本病提供实验依据。

Glaucoma is an irreversible optic neuropathy and the second leading cause of blindness worldwide. High intraocular pressure (IOP) is the main risk factor for glaucoma and can cause optic nerve injury. There is no effective treatment for this injury at home and abroad. Effective treatment drugs are urgently needed. Chinese medicine has broad prospects in this field, and has great potential to discover new compound prescriptions. Chinese medicine believes that the pathogenesis of optic nerve injury is liver and kidney deficiency, Qi deficiency and blood stasis. Based on the treatment of nourishing liver and kidney, invigorating qi and activating blood circulation, we created Qingguang An II prescription. Previous experimental studies have confirmed that Qingguang An II prescription has protective effect on chronic ocular hypertension optic nerve injury, but its specific mechanism is still unclear. Some studies have shown that there are mechanical stress receptors on the surface of retinal ganglion cell membrane and ion channel receptors as corresponding mechanical stress receptors and effectors, which are related to retinal ganglion cell apoptosis. In this study, we observed the effect of this prescription on pressure-sensitive ion channel receptors in retinal ganglion cells of rats with chronic ocular hypertension, and explored the molecular and cytological mechanism of its anti-chronic ocular hypertension optic nerve injury, so as to provide experimental basis for the treatment of this disease by traditional Chinese medicine.