肿瘤微环境中浸润的巨噬细胞通过Wnt信号通路介导的血管新生在肿瘤生长转移中起着关键作用。化痰祛瘀法是针对肺癌发病中主要病理因素“痰瘀互结”的常用和有效的治法，在稳定瘤体、延长生存期、改善生活质量等方面作用显著。前期研究显示“瓜蒌-赤芍”可阻抑血管生成，抑制肿瘤生长，延长荷瘤鼠生存期，并能调节Wnt通路相关的巨噬细胞标志性因子的表达。据此提出“化痰祛瘀法通过Wnt通路调控肿瘤相关巨噬细胞，阻抑血管新生，进而干预肺癌生长转移”的假说。课题以肺癌新生血管形成为切入点，以肿瘤相关巨噬细胞和Wnt通路之间的关系为靶点，采用体外三维新生血管形成和体内肺癌裸鼠模型，综合运用多种分子生物学技术，从整体、细胞、分子等多个层面揭示Wnt通路介导的肿瘤相关巨噬细胞促血管新生的分子机制及“瓜蒌-赤芍”的作用靶点。课题将从微环境免疫细胞调控角度揭示瓜蒌-赤芍干预血管新生的机理，为化痰祛瘀法治疗肺癌提供理论。

Macrophages in tumor microenvironment play a key role in the growth and metastasis of tumor through angiogenesis mediated by the Wnt signal pathway."Reducing phlegm and dispelling stasis method " (RPDS) is the common and effective method for the main pathological factor "phlegm and static blood combined together" in the incident of lung cancer, and it can stabilize tumor, prolong survival and improve the patient's quality of life. Our previous study confirmed that "Gualou-Chishao"(GC) can inhibit angiogenesis and tumor growth,extend the survival period of the tumor-burdened rats,and regulate the hallmark cytokine expression of macrophages in the Wnt pathway. Thus, it is hypothesized that RPDS can regulate tumor-associated macrophages to inhibit angiogenesis and intervene growth and metastasis of tumor through the Wnt pathway. We take the angiogenesis of lung cancer as a starting point, the relationship between tumor-associated macrophages and Wnt pathway as target point. By using the way of 3-D angiogenesis model in vitro and lung cancer nude mouse model in vivo and a variety of molecular biology techniques to reveal the molecular mechanism of tumor-associated macrophages promoting angiogenesis mediated by the Wnt pathway and the target points of GC from multiple levels, such as overall level, cellular level, and molecular level. This project will reveal the mechanism of angiogenesis intervened by GC from the perspective of immune cell regulation in tumor microenvironment,providing the theoretical basis and experimental evidences for the treatment of lung cancer by RPDS.