功能性便秘是临床常见病、多发病，也是目前亟待解决的临床热点问题。针灸治疗功能性便秘疗效确切，证据充分，其中大肠俞募配穴是最经典、最常用的取穴方法，然而俞募配穴治疗该病的作用机制尚未阐释清楚。前期研究表明，针刺大肠俞募配穴能有效治疗功能性便秘，而且可以提高GDNF的表达，激活PI3K-Akt通路，促进肠道神经元存活，维持ENS-ICC-SMC功能网络的完整性，从而调节胃肠活动。最新研究表明GDNF基因启动子区的甲基化修饰状态改变，能够影响其基因转录水平，是调控肠道神经细胞凋亡，调节胃肠动力的关键环节。本项目以针灸俞募配穴理论为指导，以功能性便秘模型大鼠为研究对象，从影响胃肠动力的关键环节GDNF基因启动子区甲基化修饰状态入手，运用现代多学科技术手段，探索针刺大肠俞募配穴调节胃肠功能的表观遗传调控途径，为针刺俞募配穴治疗脏腑病的作用机制及其临床应用提供坚实的科学依据。

Functional constipation (FC) is a common sickness and frequently encountered disease in the department of gastroenterology. However, effect of medication treatment is not satisfactory and a better solution is badly needed. Acupuncture has been fully proved effective in treating FC by clinical practice. Back-shu and Front-mu acupoints is a classical acupuncture approach for treating FC, but the mechanism behind has not been explained essentially.Previous studies have shown that acupuncture Back-shu and Front-mu acupoints can be effective in the treatment of FC. It was also improved that expression of GDNF can activate the PI3K-Akt pathway, survive intestinal neuronal and maintain the functional network of the ENS-ICC-SMC. Recent research seems to indicate that the methylation status of the promoter region of the GDNF changes can affect gene transcription, which is the critical path regulating gastrointestinal motility. This project starts with links of GDNF gene promoter region methylation status, which is significant factors effecting on gastrointestinal motility. Then utilizes progressive techniques to prove the regulation effect of acupuncture on GDNF gene transcription and gastrointestinal motility FC model mice pros and cons.