具有生物相容性的木素-碳水化合物复合体的分离提取及其酶催化模拟合成

木素脱氢聚合物（Dehydrogenation Polymer，简称DHP）是最接近原本木素的人工聚合物，而且其化学结构和分子量可控，DHP能在氧化酶催化脱氢反应过程中与聚糖发生接枝共聚得到具有良好理化性能的生物高分子。本研究拟模拟天然LCC的生物合成途径，建立一个高效的木素氧化酶催化脱氢聚合体系，在木素过氧化物酶和漆酶的催化作用下，以松柏醇葡萄糖苷等木素前驱物以及聚半乳糖等聚糖为起始物合成木素脱氢聚合物（DHP）-聚糖复合体，分离提取高分子量组分。分析DHP-聚糖复合体中聚糖和木素组分的含量、结构以及相互间的化学键连接，阐明脱氢聚合反应体系中木素前驱物、聚糖以及木素氧化酶的种类等因素对反应产物的得率、结构和分子量的影响。为开发性能优异的DHP-聚糖复合体生物高分子提供理论基础。

Dehydrogenation Polymer(DHP) is a synthetic polymer most similar to protolignin. Furthermore, its chemical structure and molecular weight can be well controlled. During the dehydrogenation polymerization reaction, it is possible that DHP co-polymerize with polysaccharides to obtain a biopolymer with excellent chemical and physical characteristics. In the present research, A high efficient dehydrogenation system with lignin oxidative enzyme as catalyzer will be designed to synthesize dehydrogenation polymer (DHP)-polysaccharide complexes by simulating the LCC biosynthesis route. Lignin precursor as coniferin and polysaccharide as galactan will be used as starting material to synthesize DHP-polysaccharide complexes catalyzed by lignin peroxidase and laccase. High molecular fraction will be isolated. Moreover, the chemical structure, contents and association of lignin moieties and sugar units in the complexes will also be analyzed. The effects of species and dosage of lignin precursors, polysaccharides and lignin oxidase on yield, structure and molecular weight in the dehydrogenation polymerization system will also be elucidated. The obtained information will be helpful to develop an excellent DHP-polysaccharide complexes biopolymer.