我国南方重要的森林资源之一的毛竹，磷素是其生长的重要养分因子，溶磷微生物在转化土壤难溶磷，提高磷肥利用率起到重要作用。为提高难溶性磷的转化效率，需要进一步详细研究其溶磷机制。本项目在前人研究和本课题组前期研究基础上，从溶磷微生物发挥作用巨大的根域微环境入手，选择典型毛竹林的原位根际土壤为研究对象，以16s rDNA(ITS)序列分析法结合生理生化方法进一步分析毛竹根际土壤溶磷微生物种类与生态分布，筛选出典型溶磷微生物后，进行室内培养，模拟添加磷素，采用上海同步辐射光源的动态X射线同轴位相衬度成像技术等先进技术，探明溶磷微生物细胞三维结构和磷素分布，重点揭示磷素和溶磷微生物间在微米级别上动态互作过程，进一步探明溶磷微生物溶磷机制，为毛竹林合理、高效利用磷素和根域范围内精准菌肥管理提供理论指导。

Bamboo is one of the important forest resources in South China, and phosphorus element is an important nutrient factor to the growth of bamboo. Phosphate solubilizing microorganism played an important role in transforming lowly water-soluble phosphate in soil and increasing operation rate of phosphate fertilizer. In order to improving the transformation efficiency of lowly water-soluble phosphate, we needed further study its mechanism of phosphate solubilization in detail. This project started phosphate solubilizing microorganism in which playing a huge role of root field microenvironment based on previous research and this seminar early research, selected situ rhizosphere soil of typical bamboo forest to research object, used the 16s rDNA (ITS) series analysis combine physiological and biochemical method to further analysis bamboo rhizosphere soil species and ecological distribution of phosphate solubilizing microorganism, for indoor cultivation and simulation add phosphorus after screen out the typical phosphate solubilizing microorganism. We used Shanghai dynamic X ray with in-line phase contrast imaging vanguard technology of synchrotron radiation light source to prove up phosphate solubilizing microorganism cell three dimensional structures and phosphorus distribution. Emphasis showing on dynamic mutual process in micron order between phosphorus and phosphate solubilizing microorganism, further proving the mechanism of phosphate solubilization ofphosphate solubilizing microorganism, in order to providing guidelines basis for bamboo forest reasonable and efficient using phosphorus and root field range within precision bacterial fertilizer management.