主要组织相容性复合体（MHC）在抗原递呈、诱导免疫应答等方面具有重要功能。HTLV-1作为内源性抗原，主要由MHCⅠ类分子递呈给T淋巴细胞而诱导免疫反应，因此MHCⅠ类分子在HTLV-1的感染过程中具有重要作用。而MHCⅡ类分子在此过程中发挥的作用尚不清楚。我们研究发现，在HTLV-1感染的细胞中，非经典MHCⅡ类分子HLA-DM表达升高，并与自噬蛋白LC3存在共定位现象。据此我们提出假说，HLA-DM能够通过自噬来影响HTLV-1的复制。我们拟利用共聚焦显微镜、流式细胞仪等仪器，采用免疫荧光染色、免疫共沉淀、RNAi沉默、western-blot等方法，研究HLA-DM与HTLV-1复制的关系，并探索自噬是否参与此过程。本项目将首次阐述MHCⅡ类分子HLA-DM在HTLV-1复制过程中的作用机制，不仅有利于深入理解HTLV-1的感染机理，也将为HTLV-1相关疾病的治疗提供新靶点。

The major histocompatibility complex(MHC) molecules have important functions in many aspects such as antigen presentation,induction of immune response and so on.HTLV-1,as a kind of endogenous antigen,is presented to T lymphocytes by MHC classⅠ molecules and induces immune response.So MHC classⅠmolecules are very important in HTLV-1 infection. However, the role of MHC classⅡ molecules play in this course is not clear now. We have found that the expression of HLA-DM,the non-classic MHC classⅡmolecules,is upregulated and co-localized with autophagy-associated protein LC3 in HTLV-1 infected cells.Based on above,we propose the hypothesis that HLA-DM has effect on the replication of HTLV-1 through antophagy.We will investigate the relationship between HLA-DM and the replication of HTLV-1, and explore whether autophagy plays a role in this course by the methods of immune fluorescence staining, co-immunoprecipitation, RNAi silence and western blot etc., and using the instruments of confocal micros cope and flow cytometry etc. It will be firstly revealed that mechanism of HLA-DM action to HTLV-1 replication.The result will contribute to understand HTLV-1 infection and provide new treatment target for HTLV-1 associated illnesses.