异化铁还原菌介导下典型多官能团污染物环境微界面作用机制研究

异化铁还原菌(dissimilatory iron reducing bacteria, DIRB)介导的典型多官能团有机污染物在固液界面上的分子作用机制是当前国际环境科学研究的热点和关键科学问题。尽管DIRB调控无机污染物微界面行为已取得一定进展，对于多官能团有机污染物的研究却十分有限，其难度在于缺乏原位分析的有效手段，无法在分子水平上揭示其在微生物参与下的界面反应过程。本项目将围绕氟喹诺酮类抗生素，除草剂草甘膦等典型多官能团有机污染物，DIRB和铁氧化物三元体系为研究对象，发展针对多官能团污染物的基于原位在线ATR-FTIR，表面增强拉曼等光谱技术为基础的原位动态分析平台，揭示MR-1菌在真实环境中胞外电子传递的分子机制，进而在分子水平上阐明MR-1介导下污染物在铁氧化物界面上的吸附，转化，降解机制。本研究有望在微生物介导下典型多官能团污染物的环境迁移转化及归趋等前沿环境问题取得突破。

The molecular interactions of typical multi-functional organic pollutants at solid-liquid interfaces, which is mediated by dissimilatory iron reducing bacteria, DIRB, is currently the key scientific problem in the environmental science field. Although some progress has been achieved about how DIRB participate and mediate the micro-interface behaviors and involved mechanisms of the inorganic pollutants. As for multifunctional organic pollutants, the related studied is scarce. The greatest difficulty is lack of the in situ analytical method, which restricts to reveal the microbe involved interfacial reaction process at molecular level. Thus, in this study, we plan to focus on the ternary system which composes of multifunctional organic pollutants, DIRB, and iron oxide. By developing in situ dynamic analysis platform based on ATR-FTIR and SERS, we plan to firstly reveal the molecular mechanisms of extracellular electron transport of MR-1 strain in real environment, and then explain the mechanisms of adsorption, transformation, and degradation of the multifunctional organic pollutants at the surfaces of iron oxide, which is mediated by DIRB. The results should provide the theoretical basis for further understanding of DIRB mediated environmental migration, transformation and fate of the multifunctional organic pollutants.