基于PepT1载体转运的小肽结构与氨基酸补充速率关系及机理研究

氨基酸的补充一直是人们所关注的问题，小肽（二肽和三肽）可以短时间内快速大量补充氨基酸，但是小肽（二肽和三肽）结构和氨基酸补充速率之间的关系尚不明确。因此本项目以小肽（二肽和三肽）为研究对象，重点研究PepT1载体参与的氨基酸补充过程。通过构建基于PepT1转运途径的小肽补充氨基酸的细胞模型，建立并优化氨基酸补充速率的测定方法；利用氨基酸描述符等参数化肽结构，结合神经网络等方法，探明小肽（二肽和三肽）结构与氨基酸吸收速度之间的关系；通过理论计算阐明小肽（二肽和三肽）与PepT1之间的分子结合模式。最终明确基于PepT1载体转运途径的小肽（二肽和三肽）结构和氨基酸补充速率之间的关系及机理。从而进一步完善氨基酸补充途径和机理，同时为快速补充氨基酸的小肽筛选、加工和产品开发提供新的理论依据，为食源性小肽的研究提供新的思路。

Amino acid supplementation is a key question concerned by people. Small peptides（dipeptide and tripeptide） can quickly supplement a large amount of amino acids in a short time. However, the relationship between the structure of small peptides (dipeptide and tripeptide) and the rate of amino acid supplementation is still unclear. Therefore, we take small peptides (dipeptide and tripeptide) as the starting point, focus on the amino acid supplement process involved in the PepT1 carrier. By building a cellular model based on the PepT1 transport pathway, we will establish and the method for determining the rate of small peptide (dipeptide and tripeptide) supplementing amino acids. With different amino acid descriptors and other parameters, we will establish a quantitative structure-activity relationship between the structure of small peptides (dipeptide and tripeptide) and the ratio of supplementing amino acids. We will also investigate the binding mode between the small peptides (dipeptide and tripeptide) and PepT1. This project will provide a new theoretical basis for the screening processing and developing of small peptides. This project can also provide new ideas for the research of food-derived small peptides.