基于TLR-hippo/MST信号轴探讨培土生金法调控COPD肺泡巨噬细胞骨架及免疫功能的机制研究

慢性阻塞性肺疾病（COPD）是一种复杂的慢性全身性疾病，与持续存在的慢性气道炎症相关，其中COPD频繁急性加重（AECOPD）是导致高住院率、高致死率、经济负担沉重的主要原因。研究发现肺泡巨噬细胞(AM)免疫功能缺陷是AECOPD发生发展的重要因素。基于中医培土生金的理论可以提高该病疗效，但机制尚不明确。课题组前期发现与改善AM吞噬功能密切相关，但其机理有待进一步研究，与TLR-hippo/MST信号轴调控AM是否有关，很值得深入探讨。本研究提出培土生金法可能通过调控TLR-hippo/MST信号轴，改善细胞骨架及免疫功能，减轻气道炎症，减少AECOPD发生的科学假说。采用细胞及动物为研究对象，运用培土生金法干预，检测TLR-hippo/MST信号轴、AM骨架及免疫功能，拟进一步阐释该法治疗COPD稳定期的分子机制，为中医“培土生金”治疗COPD提供客观科学依据。

Chronic obstructive pulmonary disease (COPD) is a complex chronic systemic disease associated with persistent chronic airway inflammation. The frequent acute exacerbation of COPD (AECOPD) is the main cause of high hospitalization rate, high fatality rate and heavy financial burden. It has been found that the immunodeficiency of alveolar macrophages (AM) is an important factor in the occurrence and development of AECOPD. Based on the theory of cultivating native soil and generating gold in TCM, the curative effect of this disease can be improved, but the mechanism is still unclear. Previously, our team found that AM phagocytosis was closely related to the improvement of AM phagocytosis function, but its mechanism needs further study. Whether TLR-hippo/MST signal axis regulates AM or not is worth further discussion．This study proposes a scientific hypothesis that the method of cultivating soil and generating gold may improve cytoskeleton and immune function, alleviate airway inflammation and reduce the occurrence of AECOPD by regulating TLR-hippo/MST signal axis. The TLR-hippo/MST signal axis, AM skeleton and immune function were detected by using the method of cultivating soil and generating gold，to further explain the molecular mechanism of this method in the treatment of stable COPD, and to provide objective and scientific basis for the treatment of COPD by "cultivating soil and generating gold" in TCM.