冬虫夏草由于价值高、产量小，种间相似度高，目前伪品较多，严重影响了药品的有效性与临床安全，且部分伪品与正品种间相似程度高，采用传统鉴别手段难以有效区分，加工品和粉末依靠现有腺苷含量测定等方法更是无法鉴别，目前冬虫夏草的鉴定研究已经成为一个新的研究热点。课题组在前期自然基金研究工作中首次利用双向电泳技术寻找到冬虫夏草特有的26种指标性蛋白质。本项目拟在26种指标性蛋白质中利用蛋白免疫印记和生物信息学预测初筛、生物活性验证优化可用于定量分析、且结构稳定的2-3种指标性蛋白质，进一步建立酶联免疫技术定量分析该指标性蛋白质组分的方法，建立冬虫夏草鉴定的新方法。本研究综合利用了蛋白免疫印记的高分辨、高特异的优点结合生物信息分析筛选优化指标性蛋白质和酶联免疫技术的高分辨率、重现性好、样本分析量大的特点来定量分析鉴定冬虫夏草。为动物药和种子果实类蛋白质含量高的中药材的鉴定与质量评价提供了一条新的方法。

Because of the great value and rare of Cordyceps sinensis (Berk) Sacc.,nowday the fake medicine is very much. These infuenced the validity and clinic safety, and the similarity between the true medicine and fake medicine is very close. It is very different to distinguish the medicinal materials using traditional identificated method, much more the process powder cannot distinguished. So the authentication for Cordyceps sinensis (Berk) Sacc.have turned into a study hotspot. In our preliminary studies, We found 26 kinds of indicator protein from Cordyceps. Based on these indiator protein, We designed to filter and optimization 2-3 indicator protein using Western immunoblotting and bioinformatics predicted screening, and further establish the ELISA method to do quantitative analysis of the indicator protein components for Cordyceps.Research utilization Western blot high-resolution, high-specific advantages, combining bioinformatics analysis of the filter to optimize the indicator protein and enzyme-linked immunosorbent high-resolution, good reproducibility, and quantitative analysis to identify sample analysis volume features Cordyceps sinensis. which supply a new method for the authentication of the pharmaceutical origened from animal and seeds.