油菜多室角果是一种高产相关性状，在白菜型油菜中受1个主效隐性核基因mc的控制，且该基因未被克隆。在我们前期工作中利用白菜型油菜的F2群体将mc基因精细定位到83.9kb的范围；该区段内有4个共分离标记和大约16个基因。在此基础上，本研究拟开展以下工作：1、扩大F2群体，将mc基因精细定位到40kb范围内；2、对两亲本进行转录组测序，分析重要基因的序列和表达量的差异；3、鉴定mc候选基因，在多室亲本中进行遗传互补测验；4、候选基因的时空表达与蛋白质亚细胞定位分析；5、构建MC/mc的近等基因系并进行表型观察；6、根据转录组测序结果，对候选基因相关的代谢调控基因进行表达分析，研究多室性状的遗传调控机理；7、在芥菜型和甘蓝型的多室油菜中开展mc同源基因的克隆与序列分析，研究mc基因的进化。通过上述工作，成功克隆mc基因，明确其功能，揭示多室性状形成的遗传机理，为油菜产量的遗传改良奠定理论基础。

Multilocular silique in rapeseed is considered a higher yield related trait, and it is governed by a nuclear recessive gene locus, mc, reported in Brassica rapa L, which hasn't been cloned. In previous study, we resolved mc to an 83.9-kb region in a F2 mapping population of B. rapa. And 4 markers co-segregated with the mc locus and about 16 predicted ORFs were found in the region. In the present project, we will conduct the following works: 1) fine-mapping mc to a region less than 40-kb in a larger F2 population of B. rapa; 2) to identify the variations of important genes between two parents, including the coding sequences and the gene expression level, based on RNA-seq technique; 3) to identify the candidate genes of mc, and perform the complementary test in the multilocular silique parent; 4) to clarify the temporal and spatial expression patterns and the protein subcellular localization of the candidate genes; 5) construction of near-isogenic lines of MC/mc locus for phenotypic comparison; 6) to perform gene expression analysis of mc related genes based on RNA-seq results, which will uncover the genetic regulation mechanism of multilocular silique; 7) cloning and sequence analysis of the mc homogous genes from varieties with multilocular silique in B. juncea and B. napus, which will provide the evolution information of the mc locus in Brassica. These works will help to clone mc successfully, clarify its functions and genetic regulation mechanism of mutilocular silique, which will lay a genetic foundation to the improvement of yield in rapeseed.