黄单胞菌一个新基因在致病过程中的作用的研究

黄单胞菌属病原菌是一类重要的植物病原细菌，能给农业生产带来重大的损失。分子植物病理学研究发现，黄单胞菌主要有DSF/rpf和III型分泌系统/hrp两个致病调控系统。在我们的前期工作中，鉴定了黄单胞菌一个新的致病相关基因。该基因的失活，使得病原菌基本丧失在寄主植物的致病性和在非寄主植物引起过敏反应的能力。该基因在黄单胞菌属病原菌中高度保守，但其功能此前未知，仅被注释为编码一个“保守的假定蛋白”。进一步分析发现，该基因与III型分泌系统分泌效应蛋白的效率有关。本项目拟利用基因组资源，采用现代分子生物学和生物化学技术，并结合功能基因组学研究方法和技术，对这新致病基因在十字花科黑腐病菌、水稻白叶枯病菌和水稻条斑病菌等的致病过程中的具体作用进行深入研究。研究结果将为人们了解新致病基因的功能，了解黄单胞菌致病的分子机理提供新知识。

Xanthomonas spp., a key group of plant-pathogenic bacteria, is able to cause lots of diseases in economic plants. In the past 30 years, two pathogenetic systems DSF/rpf and type III secretion/hrp were identified in Xanthomonas spp.. In our previous work, a novel gene in Xanthomonas spp., which is annotated to encode “a conserved hypothetical protein”, was identified to be required for virulence and hypersensitive reaction induction. Further research revealed that this gene plays impact on the secretion of type III secretion system effectors. To understand more about the function of the novel gene in microbes and the mechanism of pathogenicity in Xanthomonas, we will investigate the biochemical and physiological function, and the expression regulation of this gene in more detail by using the methods and techniques of modern molecular biology and functional genomics. This study will give us more knowledge about the pathogenesis of Xanthomonas spp..