睡眠剥夺（SD）导致学习记忆能力下降，神经元突触可塑性是学习记忆活动的神经生物学基础。MiR-132能促进神经细胞发育分化，调节神经元轴突生长、分支及定向，影响突触可塑性和脑认知，可能是针刺改善SD所致学习记忆能力下降的潜在生物学标志。本项目拟采用改良多平台水环境法制备大鼠SD模型，围绕“电针通过影响miR-132的表达调控p250GAP/Rac1/Cdc42信号通路，增加树棘突数量和密度，增强神经突触可塑性，改善学习记忆能力”的工作假说，运用水迷宫检测大鼠空间学习记忆能力，高尔基染色检测突触形态结构可塑性，电镜观察海马CA1区突触超微结构，膜片钳技术检测海马CA1区LTP，Western blot和RT-PCR检测miR-132、p250GAP、Rac1、Cdc42表达，从而为针灸改善SD所致学习记忆能力下降的有效干预靶点提供实验依据，为丰富针灸在脑认知领域中的作用提供新的思路和方法。

Sleep deprivation (SD) leads to the decline of learning and memory ability, and synaptic plasticity is the neurobiological basis of learning and memory. MiR-132 can promote the development and differentiation of nerve cells, regulate the axon growth, branch and orientation of neurons, and affect synaptic plasticity and brain cognition, which may be a potential biological marker to improve the ability of learning and memory induced by SD. This project intends to use modified multi platform water environment method to prepare rat SD model, work around the working hypothesis that Electroacupuncture controls the p250GAP / Rac1 / Cdc42 signaling pathway by increasing the expression of miR-132, increasing the number and density of spinous processes, enhancing synaptic plasticity, improving learning and memory. Using Morris water maze to detect spatial learning and memory ability in rats, using Golgi staining to detect synaptic morphology plasticity, using electron microscopic to observe the synaptic ultrastructure in hippocampal CA1 region, using Patch clamp technique to detect LTP in hippocampal CA1 region, using Western blot and RT-PCR to detect the expression of miR-132, p250GAP, Rac1, Cdc42, So as to provide an experimental basis for acupuncture to improve the effective intervention target of SD-induced learning and memory ability, and to provide new ideas and methods for enriching the role of acupuncture in brain cognition.